Any feedback?
Literature summary extracted from
- Iron transport by proteoliposomes containing mitochondrial F1Fo ATP synthase isolated from rat heart (2010), Biochimie, 92, 333-342.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.2 | AMP-PCP | - |
Rattus norvegicus |  |
| 7.1.2.2 | m-chlorophenylhydrazone | - |
Rattus norvegicus | |
| 7.1.2.2 | additional information | no inhibition of Fe2+ uptake and ATPase activity by ouabain at 0.1 mM, and by ATP at 1 mM | Rattus norvegicus | |
| 7.1.2.2 | oligomycin | a specific inhibitor of F1Fo ATP synthase, severely blocks transport of iron | Rattus norvegicus | |
| 7.1.2.2 | phosphate | phosphate in the medium exerted an opposite effect on iron uptake depending on the type of adenosine nucleotide, which is suppressed with ATP, but enhanced with ADP | Rattus norvegicus | |
| 7.1.2.2 | piceatanol | an F1 inhibitor, also inhibits Fe2+ uptake | Rattus norvegicus | |
| 7.1.2.2 | quercetin | an F1 inhibitor, also inhibits Fe2+ uptake | Rattus norvegicus | |
| 7.1.2.2 | resveratrol | an F1 inhibitor, also inhibits Fe2+ uptake | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.2.2 | mitochondrial membrane | - |
Rattus norvegicus | 31966 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.2 | Ca2+ | inhibits Fe2+ uptake by the enzyme by 25%, and the ATPase activity | Rattus norvegicus | |
| 7.1.2.2 | Cu2+ | inhibits Fe2+ uptake by the enzyme by 56%, and the ATPase activity | Rattus norvegicus | |
| 7.1.2.2 | Mg2+ | required for ATPase/ATP synthase activity, stimulates Fe2+ uptake activity | Rattus norvegicus | |
| 7.1.2.2 | phosphate | phosphate in the medium exerts an opposite effect on iron uptake depending on the type of adenosine nucleotide, which is suppressed with ATP, but enhanced with ADP. Mn2+ does not affect the Fe2+ uptake | Rattus norvegicus | |
| 7.1.2.2 | Zn2+ | inhibits Fe2+ uptake by the enzyme by 29%, and the ATPase activity slightly | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.2.2 | ATP + H2O + Fe2+/in | Rattus norvegicus | the enzyme transports Fe2+ and contributes to the iron uptake into rat heart. The activity of ATPase and ATP synthase may be associated with iron uptake in a different manner, probably via antiport of H+ | ADP + phosphate + Fe2+/out | - |
? | |
| 7.1.2.2 | ATP + H2O + H+/in | Rattus norvegicus | - |
ADP + phosphate + H+/out | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.2.2 | Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.2.2 | native enzyme from mitochondria | Rattus norvegicus |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 7.1.2.2 | reconstitution of the purified enzyme in proteoliposomes | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.1.2.2 | heart | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.2.2 | ATP + H2O + Fe2+/in | the enzyme transports Fe2+ and contributes to the iron uptake into rat heart. The activity of ATPase and ATP synthase may be associated with iron uptake in a different manner, probably via antiport of H+ | Rattus norvegicus | ADP + phosphate + Fe2+/out | - |
? | |
| 7.1.2.2 | ATP + H2O + H+/in | - |
Rattus norvegicus | ADP + phosphate + H+/out | - |
r | |
| 7.1.2.2 | additional information | the enzyme also performs slight transport of common divalent and trivalent metal ions such as Mg2+, Ca2+, Mn2+, Zn2+, Cu2+, Fe3+, and Al3+ | Rattus norvegicus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.2.2 | F1Fo ATP synthase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.2 | 37 | - |
optimum for Fe2+ uptake and ATPase activity | Rattus norvegicus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.2 | - |
50 | activity range for Fe2+ uptake and ATPase activity | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.2 | 8 | - |
assay at | Rattus norvegicus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.1.2.2 | physiological function | role of the F1Fo ATP synthase in iron transport, and involvement of proton-coupled transport associated with the cgamma subunit | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
