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Literature summary extracted from

  • Ishikawa, T.; Tajima, N.; Nishikawa, H.; Gao, Y.; Rapolu, M.; Shibata, H.; Sawa, Y.; Shigeoka, S.
    Euglena gracilis ascorbate peroxidase forms an intramolecular dimeric structure: its unique molecular characterization (2010), Biochem. J., 426, 125-134.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11 expressed in Escherichia coli strain BL21 Star Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.493
-
 L-ascorbate recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytosol APX becomes mature in the cytosol after processing of the precursor Euglena gracilis 5829
-
1.11.1.11 additional information not localized in plastids Euglena gracilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 30000
-
 2 * 30000, SDS-PAGE Euglena gracilis
1.11.1.11 58000
-
 mature full length APX, gel filtration Euglena gracilis
1.11.1.11 60080
-
 mature full length APX, calculated from amino acid sequence Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11 TALON metal affinity resin column chromatography Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 D-isoascorbate + H2O2 60.3% activity compared to L-ascorbate Euglena gracilis ?
-
 ?
1.11.1.11 L-ascorbate + cumene hydroperoxide 8.0% activity compared to H2O2 Euglena gracilis ?
-
 ?
1.11.1.11 L-ascorbate + H2O2 100% activity Euglena gracilis dehydroascorbate + H2O
-
 ?
1.11.1.11 L-ascorbate + tert-butyl hydroperoxide 17.4% activity compared to H2O2 Euglena gracilis ?
-
 ?
1.11.1.11 additional information no activity with guaiacol and NADPH Euglena gracilis ?
-
 ?
1.11.1.11 pyrogallol + H2O2 90.1% activity compared to L-ascorbate Euglena gracilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 homodimer 2 * 30000, SDS-PAGE Euglena gracilis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.11 APX
-
 Euglena gracilis
1.11.1.11 ascorbate peroxidase
-
 Euglena gracilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.11 577
-
 H2O2 recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis
1.11.1.11 754
-
 L-ascorbate recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.11 heme
-
 Euglena gracilis

General Information

EC Number General Information Comment Organism
1.11.1.11 physiological function APX is physiologically important to the metabolism of H2O2 Euglena gracilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.11 1530
-
 L-ascorbate recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis
1.11.1.11 16500
-
 H2O2 recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C Euglena gracilis