Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Heyen, C.A.; Tagliabracci, V.S.; Zhai, L.; Roach, P.J.
    Characterization of mouse UDP-glucose pyrophosphatase, a Nudix hydrolase encoded by the Nudt14 gene (2009), Biochem. Biophys. Res. Commun., 390, 1414-1418.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.45 expressed in Escherichia coli BL21 (DE3) cells Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.45 0.3
-
 ADP-D-ribose in 50 mM Tris-HCl, pH 8.0, 10 mM Mg2+, at 37°C Mus musculus
3.6.1.45 4.1
-
 UDP-D-glucose in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.45 Mg2+ activity is dependent on the presence of Mg2+ Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.45 26700
-
 2 * 26700, calculated from amino acid sequence Mus musculus
3.6.1.45 30000
-
 2 * 30000, SDS-PAGE Mus musculus
3.6.1.45 54000
-
 SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.45 Mus musculus Q9D142
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.45 Ni-NTA resin column chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.45 adipose tissue
-
 Mus musculus
-
3.6.1.45 brain
-
 Mus musculus
-
3.6.1.45 heart
-
 Mus musculus
-
3.6.1.45 kidney
-
 Mus musculus
-
3.6.1.45 liver
-
 Mus musculus
-
3.6.1.45 lung
-
 Mus musculus
-
3.6.1.45 pancreas
-
 Mus musculus
-
3.6.1.45 skeletal muscle
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.45 ADP-D-ribose + H2O based on Vmax/Km values, the enzyme is about 20fold more active toward ADP-D-ribose as compared to UDP-D-glucose Mus musculus AMP + alpha-D-ribose 1-phosphate
-
 ?
3.6.1.45 UDP-D-glucose + H2O
-
 Mus musculus UMP + alpha-D-glucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.45 homodimer 2 * 30000, SDS-PAGE Mus musculus
3.6.1.45 homodimer 2 * 26700, calculated from amino acid sequence Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.45 UDP-glucose pyrophosphatase
-
 Mus musculus
3.6.1.45 UGPPase
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.45 9
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.45 8 9.5
-
 Mus musculus