Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chavez-Pacheco, J.L.; Contreras-Zentella, M.; Membrillo-Hernandez, J.; Arreguin-Espinoza, R.; Mendoza-Hernandez, G.; Gomez-Manzo, S.; Escamilla, J.E.
    The quinohaemoprotein alcohol dehydrogenase from Gluconacetobacter xylinus: molecular and catalytic properties (2010), Arch. Microbiol., 192, 703-713.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.5 antimycin A powerful inhibitor of the purified ADH complex, most likely acting at the ubiquinone acceptor site in subunit II Komagataeibacter xylinus
1.1.5.5 Myxothiazol powerful inhibitor of the purified ADH complex, most likely acting at the ubiquinone acceptor site in subunit II Komagataeibacter xylinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.5 0.047
-
 ubiquinone-1 using ethanol as cosubstrate, pH and temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 0.36
-
 n-butanol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 0.43
-
 allylic alcohol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 0.66
-
 ethanol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 6.9
-
 acetaldehyde using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.5 membrane
-
 Komagataeibacter xylinus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.5 41000
-
 1 * 68000 + 1 * 41000, SDS-PAGE Komagataeibacter xylinus
1.1.5.5 68000
-
 1 * 68000 + 1 * 41000, SDS-PAGE Komagataeibacter xylinus
1.1.5.5 119000
-
 gel filtration Komagataeibacter xylinus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.5 Komagataeibacter xylinus
-
-
-
1.1.5.5 Komagataeibacter xylinus IFO 13693
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.5 QAE-Toyopearl 550C column chromatography, DEAE-Toyopearl 650 M column chromatography, HA-Ultrogel column chromatography, and Sephacryl-S200 gel filtration Komagataeibacter xylinus

Storage Stability

EC Number Storage Stability Organism
1.1.5.5 4°C, purified enzyme in 10 mM potassium phosphate buffer containing 0.1% (v/v) Triton X-100, 30 days, no appreciable loss of activity Komagataeibacter xylinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.5 acetaldehyde + 2,6-dichlorophenolindophenol 42% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus ?
-
 ?
1.1.5.5 acetaldehyde + ferricyanide 13% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus ?
-
 ?
1.1.5.5 allylic alcohol + 2,6-dichlorophenolindophenol 91% activity compared to n-butanol Komagataeibacter xylinus ?
-
 ?
1.1.5.5 allylic alcohol + ferricyanide 96% activity compared to n-butanol Komagataeibacter xylinus ?
-
 ?
1.1.5.5 ethanol + 2,6-dichlorophenolindophenol 88% activity compared to n-butanol Komagataeibacter xylinus acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.5 ethanol + 2,6-dichlorophenolindophenol 88% activity compared to n-butanol Komagataeibacter xylinus IFO 13693 acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.5 ethanol + ferricyanide 91% activity compared to n-butanol Komagataeibacter xylinus acetaldehyde + ferrocyanide
-
 ?
1.1.5.5 ethanol + ferricyanide 91% activity compared to n-butanol Komagataeibacter xylinus IFO 13693 acetaldehyde + ferrocyanide
-
 ?
1.1.5.5 ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
 Komagataeibacter xylinus ?
-
 ?
1.1.5.5 ethanol + phenazine methosulfate + 2,6-dichlorophenolindophenol
-
 Komagataeibacter xylinus IFO 13693 ?
-
 ?
1.1.5.5 ethanol + ubiquinone-1 the ADH complex shows a high affinity for ubiquinone-1 with ethanol as cosubstrate Komagataeibacter xylinus acetaldehyde + ubiquinol-1
-
 ?
1.1.5.5 formaldehyde + 2,6-dichlorophenolindophenol 38% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus ?
-
 ?
1.1.5.5 formaldehyde + ferricyanide 34% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus ?
-
 ?
1.1.5.5 glutaraldehyde + 2,6-dichlorophenolindophenol 18% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus ?
-
 ?
1.1.5.5 glutaraldehyde + ferricyanide 8% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus ?
-
 ?
1.1.5.5 glutaraldehyde + ferricyanide 8% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus IFO 13693 ?
-
 ?
1.1.5.5 additional information purified ADH oxidizes primary alcohols (C2-C6) but not methanol Komagataeibacter xylinus ?
-
 ?
1.1.5.5 additional information purified ADH oxidizes primary alcohols (C2-C6) but not methanol Komagataeibacter xylinus IFO 13693 ?
-
 ?
1.1.5.5 n-butanol + 2,6-dichlorophenolindophenol 100% activity Komagataeibacter xylinus n-butanal + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.5 n-propanol + ferricyanide 98% activity compared to n-butanol Komagataeibacter xylinus n-propanal + ferrocyanide
-
 ?
1.1.5.5 n-propanol + oxidized 2,6-dichlorophenolindophenol 96% activity compared to n-butanol Komagataeibacter xylinus n-propanal + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.5 propionaldehyde + 2,6-dichlorophenolindophenol 33% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus ?
-
 ?
1.1.5.5 propionaldehyde + ferricyanide 24% activity compared to n-butanol. The enzyme also oxidizes aldehydes, however the affinity for alcohols is at least twice as high Komagataeibacter xylinus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.5 heterodimer 1 * 68000 + 1 * 41000, SDS-PAGE Komagataeibacter xylinus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.5 ADH
-
 Komagataeibacter xylinus
1.1.5.5 quinohemoprotein alcohol dehydrogenase
-
 Komagataeibacter xylinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.5 30.8
-
 acetaldehyde using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 52.3
-
 n-butanol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 67.5
-
 allylic alcohol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 71
-
 ethanol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 76.3
-
 ubiquinone-1 using ethanol as cosubstrate, pH and temperature not specified in the publication Komagataeibacter xylinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.5 5.5
-
 activity responses to pH are sharp, showing two distinct optimal pH values (pH 5.5 and 6.5) depending on the electron acceptor used (optimum pH 5.5 with ferricyanide as electron acceptor) Komagataeibacter xylinus
1.1.5.5 6.5
-
 activity responses to pH are sharp, showing two distinct optimal pH values (pH 5.5 and 6.5) depending on the electron acceptor used (optimum pH 6.5 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are used as electron acceptors) Komagataeibacter xylinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.5 cytochrome c the enzyme contains 4 cytochromes c per enzyme Komagataeibacter xylinus
1.1.5.5 heme the quinohaemoprotein alcohol dehydrogenase contains heme C in both subunits, the ADH complex of contains 18 nmol of heme C per mg of protein (ratio of 3.6 mol of heme C per mol of enzyme) Komagataeibacter xylinus
1.1.5.5 pyrroloquinoline quinone the ADH complex contains one mol of pyrroloquinoline quinone Komagataeibacter xylinus
1.1.5.5 pyrroloquinoline quinone one pyrroloquinoline quinone is associated with one molecule of the purified ADH complex Komagataeibacter xylinus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.5.5 Komagataeibacter xylinus isoelectric focusing
-
 5.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.5.5 108
-
 ethanol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 145
-
 n-butanol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 157
-
 allylic alcohol using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 162
-
 ubiquinone-1 using ethanol as cosubstrate, pH and temperature not specified in the publication Komagataeibacter xylinus
1.1.5.5 446
-
 acetaldehyde using 2,6-dichlorophenolindophenol as cosubstrate, pH 6.5, temperature not specified in the publication Komagataeibacter xylinus