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Literature summary extracted from

  • Siggaard, J.H.; Johansson, E.; Vognsen, T.; Helt, S.S.; Harris, P.; Larsen, S.; Willemoes, M.
    Concerted bifunctionality of the dCTP deaminase-dUTPase from Methanocaldococcus jannaschii: a structural and pre-steady state kinetic analysis (2009), Arch. Biochem. Biophys., 490, 42-49.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.30 mutant enzyme E145A in complex with the substrate analogue alpha,beta-imido-dUTP and mutant enzyme E145Q in complex with diphosphate, hanging drop vapor diffusion method, using 0.2 M potassium citrate (E135Q) or 0.2 M potassium hydrogen phosphate (E145A) and 20% (w/v) PEG3350 Methanocaldococcus jannaschii
3.6.1.23 hanging drop vapor diffusion method, using 0.2 M potassium hydrogen phosphate and 20% (w/v) PEG3350 for mutant enzyme E145A complexed with alpha,beta-imido-dUTP, or 0.2 M potassium citrate and 20% (w/v) PEG3350 for mutant enzyme E145Q complexed with diphosphate Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.30 E145A the mutant enzyme is defect in the deaminase reaction Methanocaldococcus jannaschii
3.5.4.30 E145Q the mutant enzyme is defect in the deaminase reaction Methanocaldococcus jannaschii
3.6.1.23 E145A the mutant shows reduced specific activity towards dUTP Methanocaldococcus jannaschii
3.6.1.23 E145Q the mutant shows reduced specific activity towards dUTP Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.30 alpha,beta-imido-dUTP
-
Methanocaldococcus jannaschii
3.5.4.30 dTTP
-
Methanocaldococcus jannaschii
3.6.1.23 alpha,beta-imido-dUTP
-
Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required for activity Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.30 dCTP + H2O Methanocaldococcus jannaschii
-
dUMP + diphosphate + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.30 Methanocaldococcus jannaschii Q57872
-
-
3.6.1.23 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.30
-
Methanocaldococcus jannaschii
3.6.1.23
-
Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.23 0.52
-
mutant enzyme E145Q, in 50 mM HEPES, pH 6.8, at 50°C Methanocaldococcus jannaschii
3.6.1.23 1.33
-
mutant enzyme E145A, in 50 mM HEPES, pH 6.8, at 50°C Methanocaldococcus jannaschii
3.6.1.23 8.8
-
wild type enzyme, in 50 mM HEPES, pH 6.8, at 50°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.30 dCTP + H2O
-
Methanocaldococcus jannaschii dUMP + diphosphate + NH3
-
?
3.6.1.23 dUTP + H2O
-
Methanocaldococcus jannaschii dUMP + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.30 dCTP deaminase-dUTPase bifunctional enzyme Methanocaldococcus jannaschii
3.6.1.23 dCTP deaminase-dUTPase bifunctional enzyme Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
3.5.4.30 metabolism the bifunctional dCTP deaminase-dUTPase coordinates the deamination and triphosphate cleavage reactions Methanocaldococcus jannaschii