EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.99.31 | expression in Escherichia coli. Synthesis of the enzyme by different vectors with high and lower copy numbers or strong and weak promoters results in variable ratios of lycopene and neurosporene formation. The highest amount of enzyme is produced with pUC vectors with high copy number and a strong promoter. This transformant synthesizes the highest lycopene amount. The lowest enzyme concentrations is found with pPEU carrying a weak promoter. In this transformant lycopene formation is minor. With increased enzyme concentration, a proportion of lycopene higher than in Rubrivivax gelatinosus cells can be obtained | Rubrivivax gelatinosus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.31 | 15-cis-phytoene + 3 acceptor | Rubrivivax gelatinosus | - |
all-trans-neurosporene + 3 reduced acceptor | - |
? | |
1.3.99.31 | 15-cis-phytoene + 4 acceptor | Rubrivivax gelatinosus | - |
all-trans-lycopene + 4 reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.99.31 | Rubrivivax gelatinosus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.31 | 15-cis-phytoene + 3 acceptor | - |
Rubrivivax gelatinosus | all-trans-neurosporene + 3 reduced acceptor | - |
? | |
1.3.99.31 | 15-cis-phytoene + 4 acceptor | - |
Rubrivivax gelatinosus | all-trans-lycopene + 4 reduced acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.99.31 | CrtI | - |
Rubrivivax gelatinosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.99.31 | 28 | - |
assay at | Rubrivivax gelatinosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.99.31 | 8 | - |
assay at | Rubrivivax gelatinosus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.31 | FAD | added to the reaction mixture | Rubrivivax gelatinosus |