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Literature summary extracted from

  • Stickforth, P.; Sandmann, G.
    Kinetic variations determine the product pattern of phytoene desaturase from Rubrivivax gelatinosus (2007), Arch. Biochem. Biophys., 461, 235-241.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.31 expression in Escherichia coli. Synthesis of the enzyme by different vectors with high and lower copy numbers or strong and weak promoters results in variable ratios of lycopene and neurosporene formation. The highest amount of enzyme is produced with pUC vectors with high copy number and a strong promoter. This transformant synthesizes the highest lycopene amount. The lowest enzyme concentrations is found with pPEU carrying a weak promoter. In this transformant lycopene formation is minor. With increased enzyme concentration, a proportion of lycopene higher than in Rubrivivax gelatinosus cells can be obtained Rubrivivax gelatinosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.31 15-cis-phytoene + 3 acceptor Rubrivivax gelatinosus
-
all-trans-neurosporene + 3 reduced acceptor
-
?
1.3.99.31 15-cis-phytoene + 4 acceptor Rubrivivax gelatinosus
-
all-trans-lycopene + 4 reduced acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.31 Rubrivivax gelatinosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.31 15-cis-phytoene + 3 acceptor
-
Rubrivivax gelatinosus all-trans-neurosporene + 3 reduced acceptor
-
?
1.3.99.31 15-cis-phytoene + 4 acceptor
-
Rubrivivax gelatinosus all-trans-lycopene + 4 reduced acceptor
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.31 CrtI
-
Rubrivivax gelatinosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.31 28
-
assay at Rubrivivax gelatinosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.31 8
-
assay at Rubrivivax gelatinosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.31 FAD added to the reaction mixture Rubrivivax gelatinosus