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Literature summary extracted from
- Dinoflagellate luciferases: purification of luciferases from Gonyaulax polyedra, Pyrocystis lunula, and Pyrocystis fusiformis (1979), Arch. Biochem. Biophys., 196, 371-384.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.18 | Ag+ | - |
Lingulodinium polyedra |  |
| 1.13.12.18 | Ag+ | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | Ag+ | - |
Pyrocystis lunula | |
| 1.13.12.18 | chlorotetracycline | - |
Lingulodinium polyedra | |
| 1.13.12.18 | chlorotetracycline | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | chlorotetracycline | - |
Pyrocystis lunula | |
| 1.13.12.18 | Co2+ | - |
Lingulodinium polyedra | |
| 1.13.12.18 | Co2+ | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | Co2+ | - |
Pyrocystis lunula | |
| 1.13.12.18 | Cu2+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | Cu2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| 1.13.12.18 | Cu2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
| 1.13.12.18 | cyanide | - |
Lingulodinium polyedra | |
| 1.13.12.18 | cyanide | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | cyanide | - |
Pyrocystis lunula | |
| 1.13.12.18 | EDTA | - |
Lingulodinium polyedra | |
| 1.13.12.18 | EDTA | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | EDTA | - |
Pyrocystis lunula | |
| 1.13.12.18 | EGTA | - |
Lingulodinium polyedra | |
| 1.13.12.18 | EGTA | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | EGTA | - |
Pyrocystis lunula | |
| 1.13.12.18 | Fe2+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | Fe2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| 1.13.12.18 | Fe2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
| 1.13.12.18 | Fe3+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | Fe3+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| 1.13.12.18 | Fe3+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
| 1.13.12.18 | Hg2+ | - |
Lingulodinium polyedra | |
| 1.13.12.18 | Hg2+ | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | Hg2+ | - |
Pyrocystis lunula | |
| 1.13.12.18 | additional information | after a 15 min incubation at pH 8.0 and 20°C with subtilisin, trypsin, chymotrypsin, pepsin, carboxypeptidase, and leucine aminopeptidase, the flash height measured in the presence of saturating luciferin is 50% lowered and, after 1 h, the flash height is 10% of the control. Moreover, proteolysis of the extracts completely inactivates the luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | additional information | after a 15 min incubation at pH 8.0 and 20°C with subtilisin, trypsin, chymotrypsin, pepsin, carboxypeptidase, and leucine aminopeptidase, the flash height measured in the presence of saturating luciferin is 50% lowered and, after 1 h, the flash height is 10% of the control. Moreover, proteolysis of the extracts completely inactivates the luciferase | Pyrocystis fusiformis | |
| 1.13.12.18 | additional information | after a 15 min incubation at pH 8.0 and 20°C with subtilisin, trypsin, chymotrypsin, pepsin, carboxypeptidase, and leucine aminopeptidase, the flash height measured in the presence of saturating luciferin is 50% lowered and, after 1 h, the flash height is 10% of the control. Moreover, proteolysis of the extracts completely inactivates the luciferase | Pyrocystis lunula | |
| 1.13.12.18 | Ni2+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | Ni2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| 1.13.12.18 | Ni2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
| 1.13.12.18 | Pb2+ | - |
Lingulodinium polyedra | |
| 1.13.12.18 | Pb2+ | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | Pb2+ | - |
Pyrocystis lunula | |
| 1.13.12.18 | Sodium azide | - |
Lingulodinium polyedra | |
| 1.13.12.18 | Sodium azide | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | Sodium azide | - |
Pyrocystis lunula | |
| 1.13.12.18 | Sr2+ | - |
Lingulodinium polyedra | |
| 1.13.12.18 | Sr2+ | - |
Pyrocystis fusiformis | |
| 1.13.12.18 | Sr2+ | - |
Pyrocystis lunula | |
| 1.13.12.18 | Zn2+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | Zn2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| 1.13.12.18 | Zn2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.18 | Ca2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | Ca2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis lunula | |
| 1.13.12.18 | Ca2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis fusiformis | |
| 1.13.12.18 | Mg2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | Mg2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis lunula | |
| 1.13.12.18 | Mg2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis fusiformis | |
| 1.13.12.18 | Mn2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Lingulodinium polyedra | |
| 1.13.12.18 | Mn2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis lunula | |
| 1.13.12.18 | Mn2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis fusiformis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.18 | 30000 | - |
SDS-PAGE | Lingulodinium polyedra |
| 1.13.12.18 | 40000 | - |
SDS-PAGE | Pyrocystis lunula |
| 1.13.12.18 | 40000 | - |
SDS-PAGE | Pyrocystis fusiformis |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.13.12.18 | guanidine-HCl | at 18 and 5°C guanidine-HCl in the concentration range from 2-6 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Lingulodinium polyedra |
| 1.13.12.18 | guanidine-HCl | at 18 and 5°C guanidine-HCl in the concentration range from 2-6 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Pyrocystis lunula |
| 1.13.12.18 | guanidine-HCl | at 18°C and 5°C guanidine-HCl in the concentration range from 2-6 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Pyrocystis fusiformis |
| 1.13.12.18 | urea | at 18 and 5°C urea in the concentration range from 2-8 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Lingulodinium polyedra |
| 1.13.12.18 | urea | at 18 and 5°C urea in the concentration range from 2-8 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Pyrocystis lunula |
| 1.13.12.18 | urea | at 18°C and 5°C urea in the concentration range from 2-8 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Pyrocystis fusiformis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.18 | Lingulodinium polyedra | - |
formerly Gonyaulax polyedra | - |
| 1.13.12.18 | Pyrocystis fusiformis | - |
- |
- |
| 1.13.12.18 | Pyrocystis lunula | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.12.18 | ammonium sulfate precipitation, Chelex column chromatography, hydroxylapatite column chromatography, and Ultrogel gel filtration | Lingulodinium polyedra |
| 1.13.12.18 | ammonium sulfate precipitation, Chelex column chromatography, hydroxylapatite column chromatography, and Ultrogel gel filtration | Pyrocystis lunula |
| 1.13.12.18 | ammonium sulfate precipitation, Chelex column chromatography, hydroxylapatite column chromatography, and Ultrogel gel filtration | Pyrocystis fusiformis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.18 | additional information | - |
the crude extract has a specific activity of 0.000000258 at pH 8.0 and 20°C and the purified enzyme shows a specific activity of 0.00000348 quanta/min/mg at pH 8.0 and 20°C | Lingulodinium polyedra |
| 1.13.12.18 | additional information | - |
the crude extract has a specific activity of 0.000000258 at pH 8.0 and 20°C and the purified enzyme shows a specific activity of 0.00000348 quanta/min/mg at pH 8.0 and 20°C | Pyrocystis lunula |
| 1.13.12.18 | additional information | - |
the crude extract has a specific activity of 0.000000258 at pH 8.0 and 20°C and the purified enzyme shows a specific activity of 0.00000348 quanta/min/mg at pH 8.0 and 20°C | Pyrocystis fusiformis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.18 | dinoflagellate luciferin + O2 | - |
Lingulodinium polyedra | oxidized dinoflagellate luciferin + H2O + hv | - |
? | |
| 1.13.12.18 | dinoflagellate luciferin + O2 | - |
Pyrocystis lunula | oxidized dinoflagellate luciferin + H2O + hv | - |
? | |
| 1.13.12.18 | dinoflagellate luciferin + O2 | - |
Pyrocystis fusiformis | oxidized dinoflagellate luciferin + H2O + hv | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.12.18 | ? | x * 40000, SDS-PAGE | Pyrocystis lunula |
| 1.13.12.18 | ? | x * 40000, SDS-PAGE | Pyrocystis fusiformis |
| 1.13.12.18 | ? | x * 30000, SDS-PAGE | Lingulodinium polyedra |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.18 | 20 | - |
about 20°C | Lingulodinium polyedra |
| 1.13.12.18 | 20 | - |
about 20°C | Pyrocystis lunula |
| 1.13.12.18 | 20 | - |
about 20°C | Pyrocystis fusiformis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.18 | 20 | 40 | at 20°C the luciferase is progressively inactivated after a 4 h incubation time at each pH, about 50% residual activity is observed at 30°C, the enzyme appears completely inactive at 40°C and pH 7.9 and shows about 10% residual activity at 40°C and pH 6.7 | Lingulodinium polyedra |
| 1.13.12.18 | 20 | 40 | at 20°C the luciferase is progressively inactivated after a 4 h incubation time at each pH, about 50% residual activity is observed at 30°C, the enzyme appears completely inactive at 40°C and pH 7.9 and shows about 10% residual activity at 40°C and pH 6.7 | Pyrocystis lunula |
| 1.13.12.18 | 20 | 40 | at 20°C the luciferase is progressively inactivated after a 4 h incubation time at each pH, about 50% residual activity is observed at 30°C, the enzyme appears completely inactive at 40°C and pH 7.9 and shows about 10% residual activity at 40°C and pH 6.7 | Pyrocystis fusiformis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.18 | 7 | - |
- |
Lingulodinium polyedra |
| 1.13.12.18 | 7 | - |
- |
Pyrocystis lunula |
| 1.13.12.18 | 7 | - |
- |
Pyrocystis fusiformis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.18 | 6.7 | 8 | the crude enzyme extracts are more stable at pH 8.0 and 5°C in HEPES buffer than at pH 6.7 and 5°C | Lingulodinium polyedra |
| 1.13.12.18 | 6.7 | 8 | the crude enzyme extracts are more stable at pH 8.0 and 5°C in HEPES buffer than at pH 6.7 and 5°C | Pyrocystis lunula |
| 1.13.12.18 | 6.7 | 8 | the crude enzyme extracts are more stable at pH 8.0 and 5°C in HEPES buffer than at pH 6.7 and 5°C | Pyrocystis fusiformis |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.13.12.18 | Lingulodinium polyedra | isoelectric focusing | - |
5.4 |
| 1.13.12.18 | Pyrocystis lunula | isoelectric focusing | - |
5.7 |
| 1.13.12.18 | Pyrocystis fusiformis | isoelectric focusing | - |
5.7 |
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