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Literature summary extracted from
- Characteristics and biotechnological applications of microbial cholesterol oxidases (2009), Appl. Microbiol. Biotechnol., 83, 825-837.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.6 | AgNO3 | markedly inhibited by AgNO3 | Brevibacterium sterolicum |  |
| 1.1.3.6 | AgNO3 | markedly inhibited by AgNO3 | Corynebacterium cholesterolicum | |
| 1.1.3.6 | AgNO3 | markedly inhibited by AgNO3 | Streptomyces sp. | |
| 1.1.3.6 | AgNO3 | markedly inhibited by AgNO3 | Streptomyces violascens | |
| 1.1.3.6 | AgNO3 | markedly inhibited by AgNO3 | Streptoverticillium cholesterolieum | |
| 1.1.3.6 | AgNO3 | markedly inhibited by AgNO3 | uncultured Gammaproteobacteria bacterium | |
| 1.1.3.6 | butanol | the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C | Nocardia sp. | |
| 1.1.3.6 | butanol | the enzyme is inactivated by the addition of 50% (v/v) of butanol after incubation for 24 h at 37°C | Rhodococcus erythropolis | |
| 1.1.3.6 | CuSO4 | significant inhibition | Streptoverticillium cholesterolieum | |
| 1.1.3.6 | Dimethylsulfoxide | the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C | Nocardia sp. | |
| 1.1.3.6 | Dimethylsulfoxide | the enzyme is inactivated by the addition of 50% (v/v) of dimethylsulfoxide after incubation for 24 h at 37°C | Rhodococcus erythropolis | |
| 1.1.3.6 | ethyl acetate | the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C | Nocardia sp. | |
| 1.1.3.6 | ethyl acetate | the enzyme is inactivated by the addition of 50% (v/v) of ethyl acetate after incubation for 24 h at 37°C | Rhodococcus erythropolis | |
| 1.1.3.6 | Fe2+ | markedly inhibited by Fe2+ | Pseudomonas sp. | |
| 1.1.3.6 | FeCl3 | markedly inhibited by FeCl3 | Streptomyces violascens | |
| 1.1.3.6 | FeSO4 | markedly inhibited by FeSO4 | Streptomyces violascens | |
| 1.1.3.6 | Hg2+ | markedly inhibited by HgCl2 | Brevibacterium sterolicum | |
| 1.1.3.6 | Hg2+ | markedly inhibited by HgCl2 | Corynebacterium cholesterolicum | |
| 1.1.3.6 | Hg2+ | markedly inhibited by HgCl2 | Pimelobacter simplex | |
| 1.1.3.6 | Hg2+ | markedly inhibited by Hg2+ | Pseudomonas sp. | |
| 1.1.3.6 | Hg2+ | markedly inhibited by HgCl2 | Streptomyces sp. | |
| 1.1.3.6 | Hg2+ | markedly inhibited by HgCl2 | Streptomyces violascens | |
| 1.1.3.6 | Hg2+ | markedly inhibited by HgCl2 | Streptoverticillium cholesterolieum | |
| 1.1.3.6 | Hg2+ | markedly inhibited by HgCl2 | uncultured Gammaproteobacteria bacterium | |
| 1.1.3.6 | p-chloromercuribenzoic acid | the addition of p-chloromercuribenzoic acid partially reduces the activity of the enzyme | Pimelobacter simplex | |
| 1.1.3.6 | Zn2+ | markedly inhibited by Zn2+ | Pseudomonas sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.3.6 | 0.0184 | - |
cholesterol | pH 7.0, temperature not specified in the publication | Nocardia sp. | |
| 1.1.3.6 | 0.0188 | - |
cholesterol | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 1.1.3.6 | 0.0262 | - |
cholesterol | pH and temperature not specified in the publication | Chromobacterium sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.6 | Mn2+ | partially activated by the addition of Mn2+ | Pseudomonas sp. | |
| 1.1.3.6 | Mn2+ | partially activated by the addition of Mn2+ | uncultured Gammaproteobacteria bacterium | |
| 1.1.3.6 | additional information | Ag+ scarcely influences the activity of the enzyme | Chromobacterium sp. |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.6 | 53000 | - |
- |
Schizophyllum commune |
| 1.1.3.6 | 55000 | - |
- |
Streptomyces sp. |
| 1.1.3.6 | 55000 | - |
- |
Brevibacterium sterolicum |
| 1.1.3.6 | 56000 | - |
- |
Pseudomonas sp. |
| 1.1.3.6 | 56000 | - |
- |
Streptoverticillium cholesterolieum |
| 1.1.3.6 | 57000 | - |
- |
Pimelobacter simplex |
| 1.1.3.6 | 57000 | - |
- |
Corynebacterium cholesterolicum |
| 1.1.3.6 | 58000 | - |
- |
uncultured Gammaproteobacteria bacterium |
| 1.1.3.6 | 58000 | - |
- |
Streptomyces sp. |
| 1.1.3.6 | 58000 | - |
- |
Chromobacterium sp. |
| 1.1.3.6 | 60000 | - |
- |
Streptomyces fradiae |
| 1.1.3.6 | 60000 | - |
- |
Burkholderia cepacia |
| 1.1.3.6 | 63000 | - |
- |
Arthrobacter sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.6 | beta-cholestanol + O2 | Streptomyces violascens | 101% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | Brevibacterium sterolicum | 13% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | Burkholderia cepacia | 69% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | Streptomyces sp. | 75% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | Pseudomonas sp. | 78% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | uncultured Gammaproteobacteria bacterium | 79% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | Chromobacterium sp. | 95% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | Streptoverticillium cholesterolieum | 96% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | Pseudomonas sp. COX629 | 78% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | uncultured Gammaproteobacteria bacterium Y-134 | 79% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | Brevibacterium sterolicum | 20% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | uncultured Gammaproteobacteria bacterium | 29% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | Streptoverticillium cholesterolieum | 39% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | Corynebacterium cholesterolicum | 50% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | Streptomyces violascens | 64% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | Streptomyces sp. | 72% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | Chromobacterium sp. | 80% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | Burkholderia cepacia | 84% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | Burkholderia cepacia ST-200 | 84% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | uncultured Gammaproteobacteria bacterium Y-134 | 29% activity compared to cholesterol | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Brevibacterium sterolicum | 10% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Corynebacterium cholesterolicum | 12% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Streptoverticillium cholesterolieum | 17% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | uncultured Gammaproteobacteria bacterium | 32% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Chromobacterium sp. | 36% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Streptomyces sp. | 37% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Pseudomonas sp. | 40% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Pimelobacter simplex | 47% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Streptomyces violascens | 50% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Burkholderia cepacia | 59% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | Pseudomonas sp. COX629 | 40% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | uncultured Gammaproteobacteria bacterium Y-134 | 32% activity compared to cholesterol | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Pseudomonas aeruginosa | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Arthrobacter sp. | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptomyces fradiae | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Nocardia rhodochrous | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Burkholderia pseudomallei | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Rhodococcus equi | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptomyces avermitilis | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptomyces griseus | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptomyces natalensis | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptomyces sp. | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Corynebacterium urealyticum | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Nocardia farcinica | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Mycobacterium leprae | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Mycobacterium tuberculosis | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptomyces coelicolor | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Rhodococcus erythropolis | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Burkholderia thailandensis | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Paraburkholderia xenovorans | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Pseudomonas sp. | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Pimelobacter simplex | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Nocardia sp. | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Schizophyllum commune | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptomyces violascens | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Corynebacterium cholesterolicum | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | uncultured Gammaproteobacteria bacterium | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptomyces sp. | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Burkholderia cepacia | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Chromobacterium sp. | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Streptoverticillium cholesterolieum | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Brevibacterium sterolicum | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Burkholderia cepacia ST-200 | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Rhodococcus equi 103S | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Nocardia farcinica IFM 10152 | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Pseudomonas sp. COX629 | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Corynebacterium urealyticum DSM 7109 | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Mycobacterium tuberculosis H37Rv | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | Arthrobacter sp. IM79 | - |
cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | uncultured Gammaproteobacteria bacterium Y-134 | 100% activity | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Burkholderia cepacia | 16% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Streptomyces sp. | 24% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Chromobacterium sp. | 27% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Schizophyllum commune | 28% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Streptomyces violascens | 37% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Brevibacterium sterolicum | 41% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Streptoverticillium cholesterolieum | 48% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Corynebacterium cholesterolicum | 5% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | Burkholderia cepacia ST-200 | 16% activity compared to cholesterol | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | epiandrosterone + O2 | Nocardia sp. | 1% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | epiandrosterone + O2 | Burkholderia cepacia | 10% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | epiandrosterone + O2 | Chromobacterium sp. | 15% activity compared to cholesterol | ? | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Streptomyces sp. | 12% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Chromobacterium sp. | 12% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Streptomyces violascens | 14% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Pimelobacter simplex | 17% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Burkholderia cepacia | 20% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Corynebacterium cholesterolicum | 4% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Streptoverticillium cholesterolieum | 5% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Pseudomonas sp. | 51% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Burkholderia cepacia ST-200 | 20% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | Pseudomonas sp. COX629 | 51% activity compared to cholesterol | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | additional information | Pseudomonas sp. | no activity with dehydroepiandrosterone | ? | - |
? | |
| 1.1.3.6 | additional information | Schizophyllum commune | no activity with ergosterol | ? | - |
? | |
| 1.1.3.6 | additional information | Brevibacterium sterolicum | no activity with ergosterol | ? | - |
? | |
| 1.1.3.6 | additional information | uncultured Gammaproteobacteria bacterium | no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone | ? | - |
? | |
| 1.1.3.6 | additional information | Rhodococcus erythropolis | the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol | ? | - |
? | |
| 1.1.3.6 | additional information | Pseudomonas sp. COX629 | no activity with dehydroepiandrosterone | ? | - |
? | |
| 1.1.3.6 | additional information | uncultured Gammaproteobacteria bacterium Y-134 | no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone | ? | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Schizophyllum commune | 20% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Brevibacterium sterolicum | 22% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Burkholderia cepacia | 32% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Streptoverticillium cholesterolieum | 37% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Corynebacterium cholesterolicum | 38% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Streptomyces violascens | 42% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Nocardia sp. | 6% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Chromobacterium sp. | 95% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Streptomyces sp. | 99% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | Burkholderia cepacia ST-200 | 32% activity compared to cholesterol | 5-pregnen-3,20-dione + H2O2 | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.1.3.6 | Acetone | the enzyme is unstable in 50% (v/v) acetone | Chromobacterium sp. |
| 1.1.3.6 | Acetone | the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C | Chromobacterium sp. |
| 1.1.3.6 | Butanol | the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C | Chromobacterium sp. |
| 1.1.3.6 | DMSO | the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C | Chromobacterium sp. |
| 1.1.3.6 | Emal 20CM | the enzyme is relatively tolerant to treatment with Emal 20CM even at 60°C | Chromobacterium sp. |
| 1.1.3.6 | Ethanol | the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C | Chromobacterium sp. |
| 1.1.3.6 | Ethyl acetate | the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C | Chromobacterium sp. |
| 1.1.3.6 | isopropanol | the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C | Chromobacterium sp. |
| 1.1.3.6 | Methanol | the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C | Chromobacterium sp. |
| 1.1.3.6 | propan-2-ol | the activity of the enzyme is rapidly inactivated in the presence of 30% (v/v) propan-2-ol at 25°C | Brevibacterium sterolicum |
| 1.1.3.6 | SDS | the enzyme is relatively tolerant to treatment with SDS after incubation for 1 h at 30°C | Chromobacterium sp. |
| 1.1.3.6 | sodium cholate | the enzyme is stable at 50% (v/v) sodium cholate after incubation for 1 h at 30°C | Nocardia sp. |
| 1.1.3.6 | sodium cholate | the enzyme is stable in sodium cholate after incubation for 1 h at 30°C | Rhodococcus erythropolis |
| 1.1.3.6 | sodium cholate | the enzyme retains more than 80% of its original activity in 0.5% (v/v) sodium cholate after incubation for 1 h at 60°C | uncultured Gammaproteobacteria bacterium |
| 1.1.3.6 | sodium dodecyl sarcosinate | the enzyme is relatively tolerant to treatment with sodium dodecyl sarcosinate even at 60°C | Chromobacterium sp. |
| 1.1.3.6 | sodium laurylbenzenesulfonate | the enzyme is relatively tolerant to treatment with sodium laurylbenzenesulfonate after incubation for 1 h at 30°C | Chromobacterium sp. |
| 1.1.3.6 | sodium polyoxyethylene alkyl ether sulfate | the enzyme is stable at 50% (v/v) sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C | Nocardia sp. |
| 1.1.3.6 | sodium polyoxyethylene alkyl ether sulfate | the enzyme is stable in sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C | Rhodococcus erythropolis |
| 1.1.3.6 | Triton X-100 | the enzyme is stable at 50% (v/v) Triton X-100 after incubation for 1 h at 30°C | Nocardia sp. |
| 1.1.3.6 | Triton X-100 | the enzyme is stable in Triton X-100 after incubation for 1 h at 30°C | Rhodococcus erythropolis |
| 1.1.3.6 | Triton X-405 | the enzyme is stable at 50% (v/v) Triton X-405 after incubation for 1 h at 30°C | Nocardia sp. |
| 1.1.3.6 | Triton X-405 | the enzyme is stable in Triton X-405 after incubation for 1 h at 30°C | Rhodococcus erythropolis |
| 1.1.3.6 | Triton X-405 | the enzyme retains more than 80% of its original activity in 0.5% (v/v) Triton X-405 after incubation for 1 h at 60°C | uncultured Gammaproteobacteria bacterium |
| 1.1.3.6 | Tween 20 | the enzyme is stable at 50% (v/v) Tween 20 after incubation for 1 h at 30°C | Nocardia sp. |
| 1.1.3.6 | Tween 20 | the enzyme is stable in Tween 20 after incubation for 1 h at 30°C | Rhodococcus erythropolis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.3.6 | Arthrobacter sp. | - |
- |
- |
| 1.1.3.6 | Arthrobacter sp. | Q56DL0 | - |
- |
| 1.1.3.6 | Arthrobacter sp. IM79 | - |
- |
- |
| 1.1.3.6 | Brevibacterium sterolicum | P22637 | - |
- |
| 1.1.3.6 | Burkholderia cepacia | Q93RE1 | - |
- |
| 1.1.3.6 | Burkholderia cepacia ST-200 | Q93RE1 | - |
- |
| 1.1.3.6 | Burkholderia pseudomallei | - |
- |
- |
| 1.1.3.6 | Burkholderia thailandensis | Q2T0X3 | - |
- |
| 1.1.3.6 | Chromobacterium sp. | B5MGF8 | - |
- |
| 1.1.3.6 | Corynebacterium cholesterolicum | - |
- |
- |
| 1.1.3.6 | Corynebacterium urealyticum | B1VGH1 | - |
- |
| 1.1.3.6 | Corynebacterium urealyticum DSM 7109 | B1VGH1 | - |
- |
| 1.1.3.6 | Mycobacterium leprae | Q59530 | - |
- |
| 1.1.3.6 | Mycobacterium tuberculosis | P9WMV9 | - |
- |
| 1.1.3.6 | Mycobacterium tuberculosis H37Rv | P9WMV9 | - |
- |
| 1.1.3.6 | Nocardia farcinica | Q5YRJ2 | - |
- |
| 1.1.3.6 | Nocardia farcinica IFM 10152 | Q5YRJ2 | - |
- |
| 1.1.3.6 | Nocardia rhodochrous | - |
- |
- |
| 1.1.3.6 | Nocardia sp. | - |
- |
- |
| 1.1.3.6 | Paraburkholderia xenovorans | Q13UN0 | - |
- |
| 1.1.3.6 | Pimelobacter simplex | - |
- |
- |
| 1.1.3.6 | Pseudomonas aeruginosa | - |
- |
- |
| 1.1.3.6 | Pseudomonas sp. | - |
- |
- |
| 1.1.3.6 | Pseudomonas sp. COX629 | - |
- |
- |
| 1.1.3.6 | Rhodococcus equi | A0A3S5YA89 | - |
- |
| 1.1.3.6 | Rhodococcus equi 103S | A0A3S5YA89 | - |
- |
| 1.1.3.6 | Rhodococcus erythropolis | A9QAE7 | - |
- |
| 1.1.3.6 | Schizophyllum commune | - |
- |
- |
| 1.1.3.6 | Streptomyces avermitilis | Q93H76 | - |
- |
| 1.1.3.6 | Streptomyces coelicolor | Q9L0H6 | - |
- |
| 1.1.3.6 | Streptomyces fradiae | - |
- |
- |
| 1.1.3.6 | Streptomyces griseus | A7RA76 | - |
- |
| 1.1.3.6 | Streptomyces natalensis | Q9EW96 | - |
- |
| 1.1.3.6 | Streptomyces sp. | P12676 | - |
- |
| 1.1.3.6 | Streptomyces sp. | Q55020 | - |
- |
| 1.1.3.6 | Streptomyces violascens | - |
- |
- |
| 1.1.3.6 | Streptoverticillium cholesterolieum | - |
- |
- |
| 1.1.3.6 | uncultured Gammaproteobacteria bacterium | - |
- |
- |
| 1.1.3.6 | uncultured Gammaproteobacteria bacterium Y-134 | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.6 | beta-cholestanol + O2 | 101% activity compared to cholesterol | Streptomyces violascens | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 13% activity compared to cholesterol | Brevibacterium sterolicum | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 69% activity compared to cholesterol | Burkholderia cepacia | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 75% activity compared to cholesterol | Streptomyces sp. | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 78% activity compared to cholesterol | Pseudomonas sp. | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 79% activity compared to cholesterol | uncultured Gammaproteobacteria bacterium | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 95% activity compared to cholesterol | Chromobacterium sp. | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 96% activity compared to cholesterol | Streptoverticillium cholesterolieum | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 78% activity compared to cholesterol | Pseudomonas sp. COX629 | ? | - |
? | |
| 1.1.3.6 | beta-cholestanol + O2 | 79% activity compared to cholesterol | uncultured Gammaproteobacteria bacterium Y-134 | ? | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 20% activity compared to cholesterol | Brevibacterium sterolicum | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 29% activity compared to cholesterol | uncultured Gammaproteobacteria bacterium | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 39% activity compared to cholesterol | Streptoverticillium cholesterolieum | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 50% activity compared to cholesterol | Corynebacterium cholesterolicum | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 64% activity compared to cholesterol | Streptomyces violascens | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 72% activity compared to cholesterol | Streptomyces sp. | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 80% activity compared to cholesterol | Chromobacterium sp. | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 84% activity compared to cholesterol | Burkholderia cepacia | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 84% activity compared to cholesterol | Burkholderia cepacia ST-200 | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-sitosterol + O2 | 29% activity compared to cholesterol | uncultured Gammaproteobacteria bacterium Y-134 | stigmast-5-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 10% activity compared to cholesterol | Brevibacterium sterolicum | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 12% activity compared to cholesterol | Corynebacterium cholesterolicum | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 17% activity compared to cholesterol | Streptoverticillium cholesterolieum | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 32% activity compared to cholesterol | uncultured Gammaproteobacteria bacterium | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 36% activity compared to cholesterol | Chromobacterium sp. | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 37% activity compared to cholesterol | Streptomyces sp. | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 40% activity compared to cholesterol | Pseudomonas sp. | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 47% activity compared to cholesterol | Pimelobacter simplex | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 50% activity compared to cholesterol | Streptomyces violascens | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 59% activity compared to cholesterol | Burkholderia cepacia | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 40% activity compared to cholesterol | Pseudomonas sp. COX629 | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | beta-stigmasterol + O2 | 32% activity compared to cholesterol | uncultured Gammaproteobacteria bacterium Y-134 | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Pseudomonas aeruginosa | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Arthrobacter sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Streptomyces fradiae | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Nocardia rhodochrous | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Burkholderia pseudomallei | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Rhodococcus equi | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Streptomyces avermitilis | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Streptomyces griseus | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Streptomyces natalensis | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Streptomyces sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Corynebacterium urealyticum | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Nocardia farcinica | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Mycobacterium leprae | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Mycobacterium tuberculosis | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Streptomyces coelicolor | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Rhodococcus erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Burkholderia thailandensis | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Paraburkholderia xenovorans | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Pseudomonas sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Pimelobacter simplex | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Nocardia sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Schizophyllum commune | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Streptomyces violascens | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Corynebacterium cholesterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | uncultured Gammaproteobacteria bacterium | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Streptomyces sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Burkholderia cepacia | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Chromobacterium sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Streptoverticillium cholesterolieum | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Burkholderia cepacia ST-200 | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Rhodococcus equi 103S | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Nocardia farcinica IFM 10152 | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | Pseudomonas sp. COX629 | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Corynebacterium urealyticum DSM 7109 | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Mycobacterium tuberculosis H37Rv | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | - |
Arthrobacter sp. IM79 | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | cholesterol + O2 | 100% activity | uncultured Gammaproteobacteria bacterium Y-134 | cholest-4-en-3-one + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 16% activity compared to cholesterol | Burkholderia cepacia | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 24% activity compared to cholesterol | Streptomyces sp. | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 27% activity compared to cholesterol | Chromobacterium sp. | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 28% activity compared to cholesterol | Schizophyllum commune | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 37% activity compared to cholesterol | Streptomyces violascens | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 41% activity compared to cholesterol | Brevibacterium sterolicum | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 48% activity compared to cholesterol | Streptoverticillium cholesterolieum | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 5% activity compared to cholesterol | Corynebacterium cholesterolicum | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | dehydroepiandrosterone + O2 | 16% activity compared to cholesterol | Burkholderia cepacia ST-200 | androst-5-en-3,17-dione + H2O2 | - |
? | |
| 1.1.3.6 | epiandrosterone + O2 | 1% activity compared to cholesterol | Nocardia sp. | ? | - |
? | |
| 1.1.3.6 | epiandrosterone + O2 | 10% activity compared to cholesterol | Burkholderia cepacia | ? | - |
? | |
| 1.1.3.6 | epiandrosterone + O2 | 15% activity compared to cholesterol | Chromobacterium sp. | ? | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 12% activity compared to cholesterol | Streptomyces sp. | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 12% activity compared to cholesterol | Chromobacterium sp. | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 14% activity compared to cholesterol | Streptomyces violascens | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 17% activity compared to cholesterol | Pimelobacter simplex | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 20% activity compared to cholesterol | Burkholderia cepacia | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 4% activity compared to cholesterol | Corynebacterium cholesterolicum | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 5% activity compared to cholesterol | Streptoverticillium cholesterolieum | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 51% activity compared to cholesterol | Pseudomonas sp. | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 20% activity compared to cholesterol | Burkholderia cepacia ST-200 | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | ergosterol + O2 | 51% activity compared to cholesterol | Pseudomonas sp. COX629 | dehydroergosterol + H2O2 | - |
? | |
| 1.1.3.6 | additional information | no activity with dehydroepiandrosterone | Pseudomonas sp. | ? | - |
? | |
| 1.1.3.6 | additional information | no activity with ergosterol | Schizophyllum commune | ? | - |
? | |
| 1.1.3.6 | additional information | no activity with ergosterol | Brevibacterium sterolicum | ? | - |
? | |
| 1.1.3.6 | additional information | no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone | uncultured Gammaproteobacteria bacterium | ? | - |
? | |
| 1.1.3.6 | additional information | the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol | Rhodococcus erythropolis | ? | - |
? | |
| 1.1.3.6 | additional information | no activity with dehydroepiandrosterone | Pseudomonas sp. COX629 | ? | - |
? | |
| 1.1.3.6 | additional information | no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone | uncultured Gammaproteobacteria bacterium Y-134 | ? | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 20% activity compared to cholesterol | Schizophyllum commune | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 22% activity compared to cholesterol | Brevibacterium sterolicum | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 32% activity compared to cholesterol | Burkholderia cepacia | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 37% activity compared to cholesterol | Streptoverticillium cholesterolieum | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 38% activity compared to cholesterol | Corynebacterium cholesterolicum | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 42% activity compared to cholesterol | Streptomyces violascens | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 6% activity compared to cholesterol | Nocardia sp. | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 95% activity compared to cholesterol | Chromobacterium sp. | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 99% activity compared to cholesterol | Streptomyces sp. | 5-pregnen-3,20-dione + H2O2 | - |
? | |
| 1.1.3.6 | pregnenolone + O2 | 32% activity compared to cholesterol | Burkholderia cepacia ST-200 | 5-pregnen-3,20-dione + H2O2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.3.6 | CHOD | - |
Mycobacterium tuberculosis |
| 1.1.3.6 | PimE | - |
Streptomyces natalensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.6 | 30 | - |
- |
Nocardia rhodochrous |
| 1.1.3.6 | 40 | - |
- |
Rhodococcus equi |
| 1.1.3.6 | 40 | 42 | - |
Corynebacterium cholesterolicum |
| 1.1.3.6 | 40 | 50 | - |
Arthrobacter sp. |
| 1.1.3.6 | 45 | 50 | - |
Streptomyces sp. |
| 1.1.3.6 | 50 | - |
- |
Pimelobacter simplex |
| 1.1.3.6 | 50 | - |
- |
Streptomyces violascens |
| 1.1.3.6 | 50 | - |
- |
Brevibacterium sterolicum |
| 1.1.3.6 | 60 | - |
- |
Burkholderia cepacia |
| 1.1.3.6 | 65 | - |
- |
Chromobacterium sp. |
| 1.1.3.6 | 70 | - |
- |
Streptomyces fradiae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.6 | 50 | 60 | 96% activity after 30 min at 50°C, 6% activity after 30 min at 60°C | Corynebacterium cholesterolicum |
| 1.1.3.6 | 55 | - |
less than 50% activity after 15 min at 55°C | Streptomyces sp. |
| 1.1.3.6 | 60 | 85 | the enzyme retains more than 90% of its original activity after incubation for 240 min at 60°C, 90% activity after 1 h at 80°C, and 80% activity after 30 min at 85°C | Chromobacterium sp. |
| 1.1.3.6 | 70 | - |
10% activity after 1 h at 70°C | Streptomyces fradiae |
| 1.1.3.6 | 70 | 80 | 70% activity after 30 min at 70°C, complete loss of activity after 30 min at 80°C | Pseudomonas sp. |
| 1.1.3.6 | 70 | 80 | 70% residual activity after 30 min at 70°C, complete loss of activity after 30 min at 80°C | Burkholderia cepacia |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.6 | 5 | - |
- |
Schizophyllum commune |
| 1.1.3.6 | 6.5 | - |
- |
uncultured Gammaproteobacteria bacterium |
| 1.1.3.6 | 6.5 | 7 | - |
Streptomyces sp. |
| 1.1.3.6 | 6.8 | 8 | - |
Burkholderia cepacia |
| 1.1.3.6 | 7 | 7.5 | - |
Corynebacterium cholesterolicum |
| 1.1.3.6 | 7 | 7.5 | - |
Chromobacterium sp. |
| 1.1.3.6 | 7 | 7.5 | - |
Streptoverticillium cholesterolieum |
| 1.1.3.6 | 7 | - |
- |
Pseudomonas sp. |
| 1.1.3.6 | 7 | - |
- |
Nocardia sp. |
| 1.1.3.6 | 7 | - |
- |
Streptomyces fradiae |
| 1.1.3.6 | 7 | - |
- |
Nocardia rhodochrous |
| 1.1.3.6 | 7 | - |
- |
Streptomyces violascens |
| 1.1.3.6 | 7 | - |
- |
Streptomyces sp. |
| 1.1.3.6 | 7.5 | - |
- |
Pimelobacter simplex |
| 1.1.3.6 | 7.5 | - |
- |
Brevibacterium sterolicum |
| 1.1.3.6 | 7.5 | 8.5 | - |
Arthrobacter sp. |
| 1.1.3.6 | 8 | - |
- |
Rhodococcus equi |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.6 | 3 | 11 | 30°C, 1 h | Chromobacterium sp. |
| 1.1.3.6 | 4 | 8 | 25°C, 20 h | Streptomyces sp. |
| 1.1.3.6 | 4 | 10 | 37°C, 30 min | Brevibacterium sterolicum |
| 1.1.3.6 | 4 | 10 | 4°C, 4 h | Streptomyces fradiae |
| 1.1.3.6 | 4 | 11 | 30°C, 1 h | Burkholderia cepacia |
| 1.1.3.6 | 4 | 11 | 60°C, 1 min | Pseudomonas sp. |
| 1.1.3.6 | 4 | 12.5 | 22°C, 1 h | Streptoverticillium cholesterolieum |
| 1.1.3.6 | 5 | 7.5 | 60°C, 1 h | uncultured Gammaproteobacteria bacterium |
| 1.1.3.6 | 5 | 9 | 30°C, 1 h | Corynebacterium cholesterolicum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.6 | FAD | dependent on | Pseudomonas aeruginosa | |
| 1.1.3.6 | FAD | dependent on | Pseudomonas sp. | |
| 1.1.3.6 | FAD | dependent on | Pimelobacter simplex | |
| 1.1.3.6 | FAD | dependent on | Arthrobacter sp. | |
| 1.1.3.6 | FAD | dependent on | Nocardia sp. | |
| 1.1.3.6 | FAD | dependent on | Streptomyces fradiae | |
| 1.1.3.6 | FAD | dependent on | Nocardia rhodochrous | |
| 1.1.3.6 | FAD | dependent on | Schizophyllum commune | |
| 1.1.3.6 | FAD | dependent on | Streptomyces violascens | |
| 1.1.3.6 | FAD | dependent on | Corynebacterium cholesterolicum | |
| 1.1.3.6 | FAD | dependent on | Burkholderia pseudomallei | |
| 1.1.3.6 | FAD | dependent on | uncultured Gammaproteobacteria bacterium | |
| 1.1.3.6 | FAD | dependent on | Streptomyces sp. | |
| 1.1.3.6 | FAD | dependent on | Rhodococcus equi | |
| 1.1.3.6 | FAD | dependent on | Streptomyces avermitilis | |
| 1.1.3.6 | FAD | dependent on | Streptomyces griseus | |
| 1.1.3.6 | FAD | dependent on | Streptomyces natalensis | |
| 1.1.3.6 | FAD | dependent on | Corynebacterium urealyticum | |
| 1.1.3.6 | FAD | dependent on | Nocardia farcinica | |
| 1.1.3.6 | FAD | dependent on | Mycobacterium leprae | |
| 1.1.3.6 | FAD | dependent on | Mycobacterium tuberculosis | |
| 1.1.3.6 | FAD | dependent on | Streptomyces coelicolor | |
| 1.1.3.6 | FAD | dependent on | Rhodococcus erythropolis | |
| 1.1.3.6 | FAD | dependent on | Burkholderia cepacia | |
| 1.1.3.6 | FAD | dependent on | Burkholderia thailandensis | |
| 1.1.3.6 | FAD | dependent on | Paraburkholderia xenovorans | |
| 1.1.3.6 | FAD | dependent on | Chromobacterium sp. | |
| 1.1.3.6 | FAD | dependent on | Streptoverticillium cholesterolieum | |
| 1.1.3.6 | FAD | dependent on | Brevibacterium sterolicum | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.3.6 | metabolism | PimE is a key enzyme in the biosynthesis of the polyene macrolide pimaricin | Streptomyces natalensis |
| 1.1.3.6 | physiological function | ChoD plays a significant role in the pathogenesis of Mycobacterium tuberculosis | Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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