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Literature summary extracted from

  • Ahn, S.J.; Sung, J.H.; Kim, N.Y.; Lee, A.R.; Jeon, S.J.; Lee, J.S.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Molecular cloning, expression, and characterization of cathepsin L from mud loach (Misgurnus mizolepis) (2010), Appl. Biochem. Biotechnol., 162, 1858-1871.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.15 Brij 35 329.61% relative activity at 0.01% (v/v) Misgurnus mizolepis
3.4.22.15 SDS 201.87% relative activity at 0.01% (v/v) Misgurnus mizolepis
3.4.22.15 Triton X-100 208.82% relative activity at 0.05% (v/v) Misgurnus mizolepis
3.4.22.15 Tween 20 337.68% relative activity at 0.01% (v/v) Misgurnus mizolepis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.15 the pro-mature enzyme of cathepsin L is expressed in Escherichia coli DH5alphaMCR cells as a fusion protein with glutathione S-transferase in a pGEX-4 T-1 vector Misgurnus mizolepis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 1,10-phenanthroline 36.6% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 antipain 97.26% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 Aprotinin 55.22% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 chymostatin 21.29% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 Co2+ 8.01% residual activity at 5 mM Misgurnus mizolepis
3.4.22.15 Cu2+ complete inhibition at 5 mM Misgurnus mizolepis
3.4.22.15 E-64 i.e. transepoxysuccinyl-L-leucyl-amido(4-guanidino) butane, 60.1% inhibition at 0.5 mM Misgurnus mizolepis
3.4.22.15 EDTA 10.81% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 EGTA 40.55% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 Hg2+ 37.32% residual activity at 1 mM Misgurnus mizolepis
3.4.22.15 leupeptin 95.85% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 NEM 40.84% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 pepstatin A 21.54% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 phenylmethylsulfonyl fluoride 10.6% inhibition at 0.1 mM Misgurnus mizolepis
3.4.22.15 Zn2+ 14.75% residual activity at 5 mM Misgurnus mizolepis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.15 Ca2+ 202.43% relative activity at 1 mM Misgurnus mizolepis
3.4.22.15 K+ 141.16% relative activity at 1 mM Misgurnus mizolepis
3.4.22.15 Mg2+ 295.3% relative activity at 5 mM Misgurnus mizolepis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 62000
-
 recombinant GST-tagged pro-mature enzyme, SDS-PAGE Misgurnus mizolepis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.15 Gelatin + H2O Misgurnus mizolepis on gelatin-zymography, the purified pro-mature enzyme of cathepsin L is capable of hydrolyzing 0.1% gelatin at pH 7.5 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Misgurnus mizolepis A5HJW4 mud loach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15 glutathione-Sepharose column chromatography Misgurnus mizolepis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O 28.61% activity compared to benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin Misgurnus mizolepis benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O 88.77% activity compared to benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin Misgurnus mizolepis benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 benzyloxycarbonyl-L-Leu-L-Leu-L-Glu-7-amido-4-methylcoumarin + H2O 76.66% activity compared to benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin Misgurnus mizolepis benzyloxycarbonyl-L-Leu-L-Leu-L-Glu + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O 100% activity Misgurnus mizolepis benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 Gelatin + H2O on gelatin-zymography, the purified pro-mature enzyme of cathepsin L is capable of hydrolyzing 0.1% gelatin at pH 7.5 Misgurnus mizolepis ?
-
 ?
3.4.22.15 L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O 28.66% activity compared to benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin Misgurnus mizolepis L-Ala-L-Ala-L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 additional information no activity towards benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin Misgurnus mizolepis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.15 CtL
-
 Misgurnus mizolepis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.15 40
-
-
 Misgurnus mizolepis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 7.5
-
-
 Misgurnus mizolepis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.15 6 10
-
 Misgurnus mizolepis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.15 5 8.5 the enzyme remains stable at neutral and alkaline pH (8.5), its activity is greatly decreased at pH 5.0 (lysosomal pH) and below Misgurnus mizolepis