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Literature summary extracted from

  • Warrilow, A.G.; Melo, N.; Martel, C.M.; Parker, J.E.; Nes, W.D.; Kelly, S.L.; Kelly, D.E.
    Expression, purification, and characterization of Aspergillus fumigatus sterol 14-alpha demethylase (CYP51) isoenzymes A and B (2010), Antimicrob. Agents Chemother., 54, 4225-4234.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 CYP51A and CYP51B, sequence comparisons and phylogenetic tree of fungal CYP51 proteins, expression of isozymes CYP51A and CYP51B in Escherichia coli Aspergillus fumigatus

General Stability

EC Number General Stability Organism
1.14.14.154 the dithionite-reduced CO-P450 complex for purified recombinant CYP51A is unstable, rapidly denaturing to inactive P420, in marked contrast to purified recombinant CYP51B, where the CO-P450 complex is stable Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.154 additional information
-
 additional information the binding of azoles, with the exception of fluconazole, to CYP51B is tight, with the Kd values for clotrimazole, itraconazole, posaconazole, and voriconazole being 0.00021, 0.00006, 0.00012, and 0.00042 mM, respectively, and 0.004 m for fluconazole Aspergillus fumigatus
1.14.14.154 0.0031
-
 lanosterol isozyme CYP51A, pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus
1.14.14.154 0.0041
-
 eburicol isozyme CYP51A, pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus
1.14.14.154 0.0086
-
 lanosterol isozyme CYP51B, pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus
1.14.14.154 0.0226
-
 eburicol isozyme CYP51B, pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.154 membrane
-
 Aspergillus fumigatus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.154 Fe2+ heme protein Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Aspergillus fumigatus
-
 isozymes CYP51A and CYP51B, i.e. AF51A and AF51B
-
1.14.14.154 Aspergillus fumigatus Af293
-
 isozymes CYP51A and CYP51B, i.e. AF51A and AF51B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.154 recombinant isozymes CYP51A and CYP51B from Escherichia coli, the dithionite-reduced CO-P450 complex for CYP51A is unstable, rapidly denaturing to inactive P420, in marked contrast to CYP51B, where the CO-P450 complex is stable Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 eburicol + [reduced NADPH-hemoprotein reductase] + O2 isozymes CYP51A and CYP51B Aspergillus fumigatus ?
-
 ?
1.14.14.154 eburicol + [reduced NADPH-hemoprotein reductase] + O2 isozymes CYP51A and CYP51B Aspergillus fumigatus Af293 ?
-
 ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 isozymes CYP51A and CYP51B Aspergillus fumigatus ?
-
 ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 isozymes CYP51A and CYP51B Aspergillus fumigatus Af293 ?
-
 ?
1.14.14.154 additional information azole binding properties of purified CYP51A and CYP51B, overview Aspergillus fumigatus ?
-
 ?
1.14.14.154 additional information azole binding properties of purified CYP51A and CYP51B, overview Aspergillus fumigatus Af293 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51
-
 Aspergillus fumigatus
1.14.14.154 Cyp51A
-
 Aspergillus fumigatus
1.14.14.154 CYP51B
-
 Aspergillus fumigatus
1.14.14.154 sterol 14-alpha demethylase
-
 Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.154 heme two distinct azole binding conformations within the heme prosthetic group of CYP51A Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
1.14.14.154 additional information an intrinsic azole fluconazole resistance is observed in Aspergillus fumigatus Aspergillus fumigatus