EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.98.1 | analysis | protocol to measure enzyme kinetic parameters based on isothermal titration calorimetry. Presence of dimethyl sulfoxide at 10%, v/v and Triton X-100 at 0.5%, v/v seems to facilitate the substrate binding process with a small decrease in KM value | Trypanosoma cruzi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.98.1 | - |
Trypanosoma cruzi |
1.3.98.1 | expression of His-tagged enzyme | Trypanosoma cruzi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.98.1 | additional information | - |
additional information | 10% v/v dimethyl sulfoxide and 0.5% v/vTriton X-100, which seem to facilitate the substrate binding process with a small decrease in Km, enzyme-substrate complex, pseudo-first-order kinetics, thermodynamics, overview | Trypanosoma cruzi | |
1.3.98.1 | 0.00856 | - |
(S)-dihydroorotate | assay based on isothermal titration calorimetry, pH 8.0, 25°C | Trypanosoma cruzi | |
1.3.98.1 | 0.0086 | - |
L-dihydroorotate | pH not specified in the publication, temperature not specified in the publication | Trypanosoma cruzi | |
1.3.98.1 | 0.12 | - |
fumarate | pH not specified in the publication, temperature not specified in the publication | Trypanosoma cruzi | |
1.3.98.1 | 0.12 | - |
fumarate | assay based on isothermal titration calorimetry, pH 8.0, 25°C | Trypanosoma cruzi |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.98.1 | membrane | - |
Trypanosoma cruzi | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.98.1 | 34000 | - |
- |
Trypanosoma cruzi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.98.1 | L-dihydroorotate + fumarate | Trypanosoma cruzi | DHODH catalyzes a coupled redox reaction in which dihydroorotate is oxidized to orotate and fumarate is reduced to succinate | orotate + succinate | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.3.98.1 | dimethyl sulfoxide | presence of dimethyl sulfoxide at 10%, v/v and Triton X-100 at 0.5%, v/v during the assay seems to facilitate the substrate binding process with a small decrease in KM value | Trypanosoma cruzi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.98.1 | Trypanosoma cruzi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.98.1 | recombinant His-tagged enzyme by nickel affinity chromatography | Trypanosoma cruzi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.98.1 | (S)-dihydroorotate + fumarate | - |
Trypanosoma cruzi | orotate + succinate | - |
? | |
1.3.98.1 | L-dihydroorotate + fumarate | - |
Trypanosoma cruzi | orotate + succinate | - |
? | |
1.3.98.1 | L-dihydroorotate + fumarate | DHODH catalyzes a coupled redox reaction in which dihydroorotate is oxidized to orotate and fumarate is reduced to succinate | Trypanosoma cruzi | orotate + succinate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.98.1 | dimer | - |
Trypanosoma cruzi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.98.1 | DHODH | - |
Trypanosoma cruzi |
1.3.98.1 | More | a family 2 DHOD | Trypanosoma cruzi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.98.1 | 25 | - |
assay at | Trypanosoma cruzi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.98.1 | 8 | - |
assay at | Trypanosoma cruzi |