EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.3 | biotechnology | development of a method to synthesize 2-oxoglutaramate which is not commercially available and development of an enzyme assay method to measure the hydrolysis of 2-oxoglutaramate and 2-oxosuccinamate in a 96-well plate format | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.3 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 31000 | - |
SDS-PAGE | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.3 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.3 | ammonium sulfate precipitation, dialysis, DE-52 chromatography, hydroxylapatite chromatography, purified 3600fold, 15.1% recovery | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.3 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 126 | - |
2-oxoglutaramate as substrate, pH 8.5, 37°C | Rattus norvegicus |
3.5.1.3 | 570 | - |
2-oxosuccinamate as substrate, pH 8.5, 37°C | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.3 | 2-oxoglutaramate + H2O | enzyme activity measured in a 96-well plate format | Rattus norvegicus | 2-oxoglutarate + NH3 | - |
? | |
3.5.1.3 | 2-oxosuccinamate + H2O | enzyme activity measured in a 96-well plate format | Rattus norvegicus | oxaloacetate + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.3 | omega-amidase/Nit2 | omega-amidase has been shown to be identical to Nit2, a putative tumor suppressor protein | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.3 | 8.5 | - |
assay at | Rattus norvegicus |