Literature summary extracted from

Merli, A.; Manikandan, K.; Graczer, E.; Schuldt, L.; Singh, R.K.; Zavodszky, P.; Vas, M.; Weiss, M.S.
Crystallization and preliminary X-ray diffraction analysis of various enzyme-substrate complexes of isopropylmalate dehydrogenase from Thermus thermophilus (2010), Acta Crystallogr. Sect. F, 66, 738-743.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.85	expressed in Escherichia coli as a His-tagged fusion protein	Thermus thermophilus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.85	using the hanging-drop vapour diffusion method crystals of Tt-IPMDH are grown in the following combinations: Apo Tt-IPMDH (crystal diffracted to a resolution of about 1.8 A), Tt-IPMDH in complex with Mn2+ (crystal diffracted to a resolution of about 2.5 A), Tt-IPMDH in complex with Mn2+ and beta-3-isopropylmalate (crystal diffracted to a resolution of 2.2 A), Tt-IPMDH in complex with Mn2+ and NADH (crystal diffracted to a resolution of 2.5 A), and Tt-IPMDH in complex with Mn2+, beta-3-isopropylmalate and NADH (crystal diffracted to a resolution of 2.75 A)	Thermus thermophilus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.1.1.85	Mg2+	-	Thermus thermophilus
1.1.1.85	Mn2+	-	Thermus thermophilus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.85	Thermus thermophilus	Q5SIY4	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.85	using Ni-NTA chromatography	Thermus thermophilus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.85	(2R,3S)-3-isopropylmalate + NAD+	-	Thermus thermophilus	(2S)-2-isopropyl-3-oxosuccinate + NADH + H+	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.85	3-isopropylmalate dehydrogenase	-	Thermus thermophilus
1.1.1.85	Tt-beta-3-isopropylmalateDH	-	Thermus thermophilus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.85	NADH	-	Thermus thermophilus

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Release 2023.1 (January 2023)