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Literature summary extracted from

  • Chu, H.N.; Kobayashi, J.; Yoshikane, Y.; Mikami, B.; Yagi, T.
    Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti (2010), Acta Crystallogr. Sect. F, 66, 718-720.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.107 expression in Escherichia coli Mesorhizobium loti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.107 recombinant selenomethionine-substituted enzyme, crystallized by the sitting-drop vapour-diffusion method using PEG 4000 as precipitant, in the presence of 0.45 mM NAD+. The crystals diffract to 2.9 A resolution and belong to the monoclinic space group P21 Mesorhizobium loti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.107 26000
-
 x * 26000, SDS-PAGE Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.107 Mesorhizobium loti Q988B7
-
-
1.1.1.107 Mesorhizobium loti MAFF303099 Q988B7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.107 recombinant protein Mesorhizobium loti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.107 103.9
-
 selenomethionine-substituted enzyme, 30°C, pH 9.2 Mesorhizobium loti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.107 pyridoxal + NAD+
-
 Mesorhizobium loti 4-pyridoxolactone + NADH + H+
-
 ?
1.1.1.107 pyridoxal + NAD+
-
 Mesorhizobium loti MAFF303099 4-pyridoxolactone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.107 ? x * 26000, SDS-PAGE Mesorhizobium loti

Synonyms

EC Number Synonyms Comment Organism
1.1.1.107 PLDH
-
 Mesorhizobium loti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.107 NAD+
-
 Mesorhizobium loti