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Literature summary extracted from

  • Patil, D.N.; Tomar, S.; Sylvestre, M.; Kumar, P.
    Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356 (2010), Acta Crystallogr. Sect. F, 66, 1517-1520.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.56 gene bphB, overexpression of N-terminally His-tagged BphB in Escherichia coli strain BL21 (DE3) Comamonas testosteroni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.56 purified recombinant detagged BphB, sitting drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 20 mM HEPES, pH 8.0, 10% glycerol, and 300 mM NaCl, are mixed with 0.001 ml of reservoir solution containing 0.2 M sodium malonate, pH 6.0, and 20% PEG 3350, 20% glycerol is used as cryoprotectant, X-ray diffractiuon structure determination and analysis at 2.8 A resolution Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.56 29400
-
x * 29400, about Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.56 Comamonas testosteroni Q46381 gene bphB
-
1.3.1.56 Comamonas testosteroni B-356 Q46381 gene bphB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.56 recombinant His-tagged BphB from Escherichia coli strain BL21 (DE3) to homogeneity by niockel affinity chromatography, the His-tag is cleaved off followed by ultrafiltration and gel filtration Comamonas testosteroni

Subunits

EC Number Subunits Comment Organism
1.3.1.56 ? x * 29400, about Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.3.1.56 biphenyl dihydrodiol dehydrogenase
-
Comamonas testosteroni
1.3.1.56 BphB
-
Comamonas testosteroni
1.3.1.56 BphBB356
-
Comamonas testosteroni
1.3.1.56 cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase
-
Comamonas testosteroni
1.3.1.56 More the enzyme belongs to the short-chain alcohol dehydrogenase/reductase, SDR, family Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.56 NAD+ required Comamonas testosteroni