Literature summary extracted from
Patil, D.N.; Tomar, S.; Sylvestre, M.; Kumar, P.
Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356 (2010), Acta Crystallogr. Sect. F, 66, 1517-1520.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.3.1.56 |
gene bphB, overexpression of N-terminally His-tagged BphB in Escherichia coli strain BL21 (DE3) |
Comamonas testosteroni |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.3.1.56 |
purified recombinant detagged BphB, sitting drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 20 mM HEPES, pH 8.0, 10% glycerol, and 300 mM NaCl, are mixed with 0.001 ml of reservoir solution containing 0.2 M sodium malonate, pH 6.0, and 20% PEG 3350, 20% glycerol is used as cryoprotectant, X-ray diffractiuon structure determination and analysis at 2.8 A resolution |
Comamonas testosteroni |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.3.1.56 |
29400 |
- |
x * 29400, about |
Comamonas testosteroni |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.3.1.56 |
Comamonas testosteroni |
Q46381 |
gene bphB |
- |
1.3.1.56 |
Comamonas testosteroni B-356 |
Q46381 |
gene bphB |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.3.1.56 |
recombinant His-tagged BphB from Escherichia coli strain BL21 (DE3) to homogeneity by niockel affinity chromatography, the His-tag is cleaved off followed by ultrafiltration and gel filtration |
Comamonas testosteroni |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.3.1.56 |
? |
x * 29400, about |
Comamonas testosteroni |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.3.1.56 |
biphenyl dihydrodiol dehydrogenase |
- |
Comamonas testosteroni |
1.3.1.56 |
BphB |
- |
Comamonas testosteroni |
1.3.1.56 |
BphBB356 |
- |
Comamonas testosteroni |
1.3.1.56 |
cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase |
- |
Comamonas testosteroni |
1.3.1.56 |
More |
the enzyme belongs to the short-chain alcohol dehydrogenase/reductase, SDR, family |
Comamonas testosteroni |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.3.1.56 |
NAD+ |
required |
Comamonas testosteroni |
|