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Literature summary extracted from

  • Sendovski, M.; Kanteev, M.; Shuster Ben-Yosef, V.; Adir, N.; Fishman, A.
    Crystallization and preliminary X-ray crystallographic analysis of a bacterial tyrosinase from Bacillus megaterium (2010), Acta Crystallogr. Sect. F, 66, 1101-1103.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.1 expressed in Escherichia coli BL21(DE3) cells Priestia megaterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.18.1 hanging drop vapor diffusion method, using 18% (w/v) PEG 8000, 0.2 M zinc acetate, 0.1 M cacodylic acid pH 5.6-6.1, at 16°C Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 Cu2+ a type 3 copper enzyme possessing two copper ions in the active site Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 35000
-
 SDS-PAGE Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 Ni2+-bound affinity column chromatography and AEC-HPLC column chromatography Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-DOPA + O2
-
 Priestia megaterium dopaquinone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 tyr
-
 Priestia megaterium
1.14.18.1 tyrosinase
-
 Priestia megaterium