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Literature summary extracted from

  • Domenech, J.; Baker, P.; Sedelnikova, S.; Rodgers, H.; Rice, D.; Ferrer, J.
    Crystallization and preliminary X-ray analysis of D-2-hydroxyacid dehydrogenase from Haloferax mediterranei (2009), Acta Crystallogr. Sect. F, 65, 415-418.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.272 expression in Escherichia coli Haloferax mediterranei
1.1.1.345 expressed in Escherichia coli BL21(DE3) cells Haloferax mediterranei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.272 free enzyme, to 3.0 A resolution, and the nonproductive ternary complex with alpha-ketohexanoic acid and NAD+ to 2.0 A resolution, by hanging-drop vapour-diffusion method Haloferax mediterranei
1.1.1.345 hanging drop vapor diffusion method, using 2.0-3.5 M ammonium sulfate or 14-26% PEG 3350 as precipitant Haloferax mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.345 33000
-
 x * 33000, SDS-PAGE Haloferax mediterranei

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.272 Haloferax mediterranei Q2VEQ7
-
-
1.1.1.345 Haloferax mediterranei
-
-
-
1.1.1.345 Haloferax mediterranei ATCC 335500
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.272 recombinant enzyme Haloferax mediterranei
1.1.1.345
-
 Haloferax mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.345 2-oxocarboxylate + NADH + H+
-
 Haloferax mediterranei (R)-2-hydroxycarboxylate + NAD+
-
 r
1.1.1.345 2-oxocarboxylate + NADH + H+
-
 Haloferax mediterranei ATCC 335500 (R)-2-hydroxycarboxylate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.345 ? x * 33000, SDS-PAGE Haloferax mediterranei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.345 D-2-hydroxyacid dehydrogenase
-
 Haloferax mediterranei
1.1.1.345 D2-HDH
-
 Haloferax mediterranei