EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.93 | expression in Escherichia coli | Pseudomonas putida |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.93 | in complex with the intermediate analog oxalate, Mg2+ and NADH, to 2.0 A resolution | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.93 | D225A | mutation in metal ion-binding ligand, 20fold decrease in metal ion-binding affinity and a two-orders-of-magnitude decrease in catalysis | Pseudomonas putida |
1.1.1.93 | D250A | mutation in metal ion-binding ligand, 10fold decrease in metal ion-binding affinity and a two-orders-of-magnitude decrease in catalysis | Pseudomonas putida |
1.1.1.93 | R108L | 1.3% of wild-type catalytic rate | Pseudomonas putida |
1.1.1.93 | R108Q | 8.7% of wild-type catalytic rate | Pseudomonas putida |
1.1.1.93 | R98L | mutant is not expressed in stable form | Pseudomonas putida |
1.1.1.93 | R98Q | 2.6% of wild-type catalytic rate | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.93 | 0.025 | - |
NAD+ | wild-type, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.079 | - |
D-malate | wild-type, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.093 | - |
D-malate | mutant R108Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.096 | - |
NAD+ | mutant R98Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.098 | - |
D-malate | mutant R98Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.135 | - |
NAD+ | mutant R108Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.18 | - |
D-malate | mutant R108L, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.24 | - |
NAD+ | mutant R108L, pH 7.5, temperature not specified in the publication | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.93 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.93 | recombinant enzyme | Pseudomonas putida |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.93 | tartrate + NAD+ = oxaloglycolate + NADH + H+ | lysyl amino group, Lys192 is the base responsible for the water-mediated proton abstraction from the C2 hydroxyl group of the substrate that begins the catalytic reaction, followed by hydride transfer to NAD. The hydroxyl group of Tyr141 functions as a general acid to protonate the enolate intermediate. Each substrate undergoes the initial hydride transfer | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.93 | D-malate + NAD+ | - |
Pseudomonas putida | pyruvate + CO2 + NADH + H+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.93 | 0.015 | - |
NAD+ | mutant D225A, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.06 | - |
NAD+ | mutant R108Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.087 | - |
NAD+ | mutant R108L, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.088 | - |
NAD+ | mutant D250A, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.175 | - |
NAD+ | mutant R98Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 6.5 | - |
NAD+ | wild-type, pH 7.5, temperature not specified in the publication | Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.93 | 0.36 | - |
NAD+ | mutant R108L, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 0.48 | - |
D-malate | mutant R108L, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 1.8 | - |
NAD+ | mutant R98Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 1.8 | - |
D-malate | mutant R98Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 4.3 | - |
NAD+ | mutant R108Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 6.2 | - |
D-malate | mutant R108Q, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 82 | - |
D-malate | wild-type, pH 7.5, temperature not specified in the publication | Pseudomonas putida | |
1.1.1.93 | 260 | - |
NAD+ | wild-type, pH 7.5, temperature not specified in the publication | Pseudomonas putida |