BRENDA - Enzyme Database show

Expansion of the aspartate beta-semialdehyde dehydrogenase family: the first structure of a fungal ortholog

Arachea, B.T.; Liu, X.; Pavlovsky, A.G.; Viola, R.E.; Acta Crystallogr. Sect. D 66, 205-212 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
expressed in Escherichia coli BL21 cells
Candida albicans
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.2.1.11
hanging drop vapor diffusion method, using 20% (w/v) PEG 400, 0.1 M HEPES pH 6.5, 0.1 M MgCl2, at 20°C
Candida albicans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Candida albicans
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Candida albicans
Q5ALM0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
cobalt immobilized metal-affinity column chromatography and Source 30Q column chromatography
Candida albicans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
710717
Candida albicans
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
homodimer
x-ray crystallography
Candida albicans
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.12
-
L-aspartate 4-semialdehyde
pH and temperature not specified in the publication
Candida albicans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Candida albicans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
expressed in Escherichia coli BL21 cells
Candida albicans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Candida albicans
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.2.1.11
hanging drop vapor diffusion method, using 20% (w/v) PEG 400, 0.1 M HEPES pH 6.5, 0.1 M MgCl2, at 20°C
Candida albicans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Candida albicans
-
L-4-aspartyl phosphate + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
cobalt immobilized metal-affinity column chromatography and Source 30Q column chromatography
Candida albicans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
710717
Candida albicans
L-4-aspartyl phosphate + NADPH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
homodimer
x-ray crystallography
Candida albicans
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.12
-
L-aspartate 4-semialdehyde
pH and temperature not specified in the publication
Candida albicans