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Literature summary extracted from

  • Gomes Junior, J.E.; Souza, D.S.; Nascimento, R.M.; Lima, A.L.; Melo, J.A.; Rocha, T.L.; Miller, R.N.; Franco, O.L.; Grossi-de-Sa, M.F.; Abreu, L.R.
    Purification and characterization of a liver-derived beta-N-acetylhexosaminidase from marine mammal Sotalia fluviatilis (2010), Protein J., 29, 188-194.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 chondroitin 6-sulfate 51% activation at 3 mM Sotalia fluviatilis
3.2.1.52 D-fructose 58% activation at 3 mM Sotalia fluviatilis
3.2.1.52 D-galactose 80% activation at 3 mM Sotalia fluviatilis
3.2.1.52 D-glucose 64% activation at 3 mM Sotalia fluviatilis
3.2.1.52 D-glucuronic acid 65% activation at 3 mM Sotalia fluviatilis
3.2.1.52 diethyldicarbonate 37% activation at 1 mM Sotalia fluviatilis
3.2.1.52 heparin 60% activation at 3 mM Sotalia fluviatilis
3.2.1.52 lactose 71% activation at 3 mM Sotalia fluviatilis
3.2.1.52 maltose 56% activation at 3 mM Sotalia fluviatilis
3.2.1.52 N-acetylgalactosamine 9% activation at 3 mM Sotalia fluviatilis
3.2.1.52 N-acetylglucosamine 38% activation at 3 mM Sotalia fluviatilis
3.2.1.52 N-ethylmaleimide 38% activation at 1 mM Sotalia fluviatilis
3.2.1.52 PMSF 74% activation at 1 mM Sotalia fluviatilis
3.2.1.52 sucrose 44% activation at 3 mM Sotalia fluviatilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 fucoidan 88% inhibition at 3 mM Sotalia fluviatilis
3.2.1.52 HgCl2 70% inhibition at 1 mM Sotalia fluviatilis
3.2.1.52 Na2S2O3 9% inhibition at 1 mM Sotalia fluviatilis
3.2.1.52 SDS 21% inhibition at 1 mM Sotalia fluviatilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.52 Ag+ activates 15% at 1 mM Sotalia fluviatilis
3.2.1.52 Ba2+ activates 22% at 1 mM Sotalia fluviatilis
3.2.1.52 Ca2+ activates 22% at 1 mM Sotalia fluviatilis
3.2.1.52 Cu2+ activates 13% at 1 mM Sotalia fluviatilis
3.2.1.52 Li+ activates 20% at 1 mM Sotalia fluviatilis
3.2.1.52 additional information no effect by 1 mM iodoacetamide Sotalia fluviatilis
3.2.1.52 Na2SeO4 activates 16% at 1 mM Sotalia fluviatilis
3.2.1.52 phosphate activates 17% at 1 mM Sotalia fluviatilis
3.2.1.52 Zn2+ activates 33% at 1 mM Sotalia fluviatilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 10077
-
 x * 10077, SDS-PAGE and mass spectrometry Sotalia fluviatilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Sotalia fluviatilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 native enzyme 380.5fold from liver by ammonium sulfate fractionation, by gel filtration, and by chitin affinity, anion exchange, and hydroxy apatite chromatography Sotalia fluviatilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 liver
-
 Sotalia fluviatilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 22.25
-
 purified native enzyme Sotalia fluviatilis

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 10077, SDS-PAGE and mass spectrometry Sotalia fluviatilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 60
-
-
 Sotalia fluviatilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5
-
-
 Sotalia fluviatilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 3 8.5
-
 Sotalia fluviatilis