EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.99.18 | functionally expressed in Escherichia coli | Campylobacter jejuni |
2.4.99.19 | gene pglB, functional expression in Escherichia coli strains E69, CWG28, or CWG44 using an arabinose-inducible promoter, coexpression of the Acra protein substrate | Campylobacter jejuni |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.99.19 | additional information | functional transfer of the Campyobacter jejuni glycosylation system into Salmonella enterica | Campylobacter jejuni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter jejuni | - |
dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine | - |
? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter jejuni | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.99.18 | Campylobacter jejuni | - |
- |
- |
2.4.99.19 | Campylobacter jejuni | - |
gene pglB | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter jejuni | dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine | - |
? | |
2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine | to investigate the substrate specificity of PglB, the transfer of a set of lipid-linked polysaccharides in Escherichia coli and Salmonella enterica serovar typhimurium is tested. A hexose linked to the C-6 of the monosaccharide at the reducing end does not inhibit the transfer of the O antigen to the acceptor protein. PglB requires an acetamido group at the C-2. A model for the mechanism of PglB involving this functional group is proposed. Eukaryotic and prokaryotic OTases catalyze the transfer of oligosaccharides by a conserved mechanism. Substitution at the C-6 position in the reducing end of the oligosaccharide does not prevent its PglB-mediated transfer to the protein acceptor AcrA. PglB transferrs a polysaccharide that is assembled by a Wzy-protein-independent pathway. An N-acetyl group in position 2 of the sugar directly linked to the undecaprenyl diphosphate carrier is necessary for recognition and/or catalysis | Campylobacter jejuni | dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine | - |
? | |
2.4.99.19 | additional information | the PglB relaxed oligosaccharide substrate specificity allows the transfer of different glycans from the lipid carrier undecaprenyl pyrophosphate to an acceptor protein, substrate specificity of PglB with a set of lipid-linked polysaccharides in Escherichia coli and Salmonella enterica serovar Typhimurium. PglB required an acetamido group at the C-2, while a hexose linked to the C-6 of the monosaccharide at the reducing end does not inhibit the transfer of the O antigen to the acceptor protein, mechanism of PglB, modeling, overview. The Salmonella enterica O antigen containing Gal at the reducing end , and K30 capsular antigen, and LT2 antigen are not substrates for PglB, as | Campylobacter jejuni | ? | - |
? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphospho-O1 antigen + [protein]-L-asparagine | from Shigella dysenteriae | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O1 antigen | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphospho-O11 antigen + [protein]-L-asparagine | from Pseudomonas aeruginosa | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O11 antigen | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphospho-O157 antigen + [protein]-L-asparagine | from Escherichia coli | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O157 antigen | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphospho-O16 antigen + [protein]-L-asparagine | from Escherichia coli | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O16 antigen | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphospho-O7 antigen + [protein]-L-asparagine | from Escherichia coli | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-O7 antigen | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphospho-O9 antigen + [Acra]-L-asparagine | PglB transfers the O9a antigen, an ABC transporter-dependent O antigen, to AcrA in Escherichia coli strain CWG28, the K30 antigen is not transferred to AcrA | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [Acra]-L-asparagine-O9 antigen | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.99.18 | OTase | - |
Campylobacter jejuni |
2.4.99.18 | PglB | - |
Campylobacter jejuni |
2.4.99.18 | PglB oligosaccharyltransferase | - |
Campylobacter jejuni |
2.4.99.19 | bacterial oligosaccharyltransferase | - |
Campylobacter jejuni |
2.4.99.19 | oligosacharyltransferase | - |
Campylobacter jejuni |
2.4.99.19 | OTase | - |
Campylobacter jejuni |
2.4.99.19 | PglB | - |
Campylobacter jejuni |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.99.19 | evolution | eukaryotic and prokaryotic OTases catalyze the transfer of oligosaccharides by a conserved mechanism | Campylobacter jejuni |