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Literature summary extracted from

  • D'Auria, J.C.; Chen, F.; Pichersky, E.
    Characterization of an acyltransferase capable of synthesizing benzyl benzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri (2002), Plant Physiol., 130, 466-476.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.195 expression in Escherichia coli Arabidopsis thaliana
2.3.1.196 expression in Escherichia coli Clarkia breweri
2.3.1.196 expression in Escherichia coli Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.196 Ca2+ 5 mM, strong inhibition Clarkia breweri
2.3.1.196 Co2+ 5 mM, strong inhibition Clarkia breweri
2.3.1.196 Cu2+ 5 mM, strong inhibition Clarkia breweri
2.3.1.196 Mg2+ 5 mM, strong inhibition Clarkia breweri
2.3.1.196 Zn2+ 5 mM, strong inhibition Clarkia breweri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.195 0.0105
-
 acetyl-CoA pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.195 0.165
-
 (3Z)-hex-3-en-ol pH 7.7, 25°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.196 0.019
-
 benzyl alcohol pH 7.7, 22°C, cosustrate: benzoyl-CoA Nicotiana tabacum
2.3.1.196 0.0205
-
 benzoyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri
2.3.1.196 0.035
-
 benzoyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Nicotiana tabacum
2.3.1.196 0.0468
-
 benzyl alcohol pH 7.7, 22°C, cosustrate: benzoyl-CoA Clarkia breweri
2.3.1.196 0.0978
-
 cinnamyl alcohol pH 7.7, 22°C, cosustrate: benzoyl-CoA Clarkia breweri
2.3.1.196 0.464
-
 cinnamoyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri
2.3.1.196 0.818
-
 acetyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.195 50290
-
 calculated from sequence Arabidopsis thaliana
2.3.1.196 47000 49500 gel filtration Clarkia breweri
2.3.1.196 50650
-
 calculated from sequence Clarkia breweri
2.3.1.196 51010
-
 calculated from sequence Nicotiana tabacum
2.3.1.196 51014
-
 x * 51014, calculated from sequence Nicotiana tabacum
2.3.1.196 55000
-
 1 * 55000, SDS-PAGE Clarkia breweri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.195 acetyl-CoA + (3Z)-hex-3-en-ol Arabidopsis thaliana
-
 (Z)-3-hexen-1-yl acetate + coenzyme A
-
 ?
2.3.1.196 benzoyl-CoA + benzyl alcohol Clarkia breweri the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma benzyl benzoate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.195 Arabidopsis thaliana Q9SRQ2
-
-
2.3.1.196 Clarkia breweri Q8GT21
-
-
2.3.1.196 Nicotiana tabacum Q8GT20
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.196 complete, non-fusion BEBT enzyme from the crude Escherichia coli extract Clarkia breweri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.196 flower the BEBT gene is expressed in different parts of the flowers with maximal RNA transcript levels in the stigma Clarkia breweri
-
2.3.1.196 leaf induced upon wounding Nicotiana tabacum
-
2.3.1.196 leaf no expression in the leaves under normal conditions. BEBT expression is induced in damaged leaves, reaching a maximum 6 h after damage occurres Clarkia breweri
-
2.3.1.196 petal 30% of the activity in stigma Clarkia breweri
-
2.3.1.196 sepal 30% of the activity in stigma Clarkia breweri
-
2.3.1.196 stamen 30% of the activity in stigma Clarkia breweri
-
2.3.1.196 stigma the BEBT gene is expressed in different parts of the flowers with maximal RNA transcript levels in the stigma Clarkia breweri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.195 acetyl-CoA + (3Z)-hex-3-en-ol
-
 Arabidopsis thaliana (3Z)-hex-3-en-1-yl acetate + CoA
-
 ?
2.3.1.195 acetyl-CoA + (3Z)-hex-3-en-ol
-
 Arabidopsis thaliana (Z)-3-hexen-1-yl acetate + coenzyme A
-
 ?
2.3.1.196 acetyl-CoA + benzyl alcohol the Km-value for acetyl-CoA (0.818 mM) strongly suggests that acetyl-CoA is not commonly used by BEBT as the acyl donor in vivo Clarkia breweri benzyl acetate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + benzyl alcohol
-
 Clarkia breweri benzyl benzoate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + benzyl alcohol
-
 Nicotiana tabacum benzyl benzoate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + benzyl alcohol the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma Clarkia breweri benzyl benzoate + CoA
-
 ?
2.3.1.196 benzoyl-CoA + cinnamyl alcohol
-
 Clarkia breweri cinnamyl benzoate + CoA
-
 ?
2.3.1.196 cinnamoyl-CoA + benzyl alcohol the Km-value for cinnamoyl-CoA (0.464 mM) strongly suggests that acetyl-CoA is not commonly used by BEBT as the acyl donor in vivo Clarkia breweri ? + CoA
-
 ?
2.3.1.196 additional information coumaroyl-CoA does not act as substrate Clarkia breweri ?
-
 ?
2.3.1.196 additional information no activity with acetyl-CoA at concentrations below 1 mM Nicotiana tabacum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.195 ? x * 50290, calculated from sequence Arabidopsis thaliana
2.3.1.196 ? x * 51014, calculated from sequence Nicotiana tabacum
2.3.1.196 monomer 1 * 55000, SDS-PAGE Clarkia breweri
2.3.1.196 monomer 1 * 50650, calculated from sequence Clarkia breweri

Synonyms

EC Number Synonyms Comment Organism
2.3.1.195 ChAT
-
 Arabidopsis thaliana
2.3.1.196 BEBT
-
 Clarkia breweri
2.3.1.196 benzoyl-CoA:benzyl alcohol benzoyl transferase
-
 Clarkia breweri
2.3.1.196 benzoyl-coenzyme A:benzyl alcohol benzoyl transferase
-
 Clarkia breweri
2.3.1.196 HSR201
-
 Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.195 22
-
 assay at Arabidopsis thaliana
2.3.1.196 22
-
 assay at Clarkia breweri
2.3.1.196 22
-
 assay at Nicotiana tabacum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.196 30
-
 30 min, stable Clarkia breweri
2.3.1.196 37
-
 30 min, 20% loss of activity Clarkia breweri
2.3.1.196 50
-
 30 min, complete inactivation Clarkia breweri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.196 0.9
-
 cinnamoyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri
2.3.1.196 21.5
-
 acetyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri
2.3.1.196 47.2
-
 cinnamyl alcohol pH 7.7, 22°C, cosustrate: benzoyl-CoA Clarkia breweri
2.3.1.196 85.2
-
 benzyl alcohol pH 7.7, 22°C, cosustrate: benzoyl-CoA Clarkia breweri
2.3.1.196 124
-
 benzoyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.195 7.7
-
-
 Arabidopsis thaliana
2.3.1.196 7.7
-
-
 Clarkia breweri
2.3.1.196 7.7
-
 assay at Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.196 6.5 9 pH 6.5: 39% of maximal activity, pH 9.0: 69% of maximal activity Clarkia breweri

General Information

EC Number General Information Comment Organism
2.3.1.196 physiological function the enzyme catalyzes the formation of benzyl benzoate, a minor constituent of the Clarkia breweri floral aroma Clarkia breweri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.196 0.4
-
 cinnamoyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri
2.3.1.196 2.5
-
 benzoyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri
2.3.1.196 4
-
 benzyl alcohol pH 7.7, 22°C, cosustrate: benzoyl-CoA Clarkia breweri
2.3.1.196 4.6
-
 cinnamyl alcohol pH 7.7, 22°C, cosustrate: benzoyl-CoA Clarkia breweri
2.3.1.196 17.5
-
 acetyl-CoA pH 7.7, 22°C, cosustrate: benzyl alcohol Clarkia breweri