EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.3.5 | gene FCLY, encoded on chromosome 5, DNAS and amino acid sequence determination and analysis | Arabidopsis thaliana |
1.8.3.6 | gene FCLY, encoded on chromosome 5, DNA and amino acid sequence determination and analysis | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.3.5 | additional information | T-DNA insertions mutants fcly-1 and fcly-2 plants exhibit reduced FCLY mRNA levels, as determined by semi-quantitative RT-PCR, and reduced FC lyase activity compared with Columbia (Col-0) and wild-type siblings. Significantly, germination of fcly-1 and fcly-2 seeds is inhibited by exogenous ABA at concentrations that did not affect wild-type Col-0 seeds | Arabidopsis thaliana |
1.8.3.6 | additional information | T-DNA insertions mutants fcly-1 and fcly-2 plants exhibit reduced FCLY mRNA levels, as determined by semi-quantitative RT-PCR, and reduced FC lyase activity compared with Columbia (Col-0) and wild-type siblings. Significantly, germination of fcly-1 and fcly-2 seeds is inhibited by exogenous ABA at concentrations that did not affect wild-type Col-0 seeds | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.3.6 | additional information | S-geranyl-L-cysteine or S-geranylgeranyl-L-cysteine fail to inhibit S-farnesyl-L-cysteine lyase | Arabidopsis thaliana | |
1.8.3.6 | N-acetyl-S-farnesyl-L-cysteine | a 50fold molar excess of unlabeled N-acetyl-S-farnesyl-L-cysteine inhibits the conversion of [1-3H]-S-farnesyl-L-cysteine to [1-3H]-farnesal by more than 50% | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.3.5 | membrane | - |
Arabidopsis thaliana | 16020 | - |
1.8.3.6 | membrane | - |
Arabidopsis thaliana | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.3.5 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | Arabidopsis thaliana | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? | |
1.8.3.5 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | Arabidopsis thaliana Col-0 | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? | |
1.8.3.6 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | Arabidopsis thaliana | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? | |
1.8.3.6 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | Arabidopsis thaliana Col-0 | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.3.5 | Arabidopsis thaliana | P57681 | gene FCLY | - |
1.8.3.5 | Arabidopsis thaliana Col-0 | P57681 | gene FCLY | - |
1.8.3.6 | Arabidopsis thaliana | P57681 | - |
- |
1.8.3.6 | Arabidopsis thaliana | P57681 | gene FCLY | - |
1.8.3.6 | Arabidopsis thaliana Col-0 | P57681 | gene FCLY | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.3.5 | additional information | the enzyme is ubiquitously expressed in Arabidopsis tissues and organs | Arabidopsis thaliana | - |
1.8.3.6 | flower | ubiquitously expressed in Arabidopsis tissues and organs | Arabidopsis thaliana | - |
1.8.3.6 | leaf | ubiquitously expressed in Arabidopsis tissues and organs | Arabidopsis thaliana | - |
1.8.3.6 | additional information | the enzyme is ubiquitously expressed in Arabidopsis tissues and organs | Arabidopsis thaliana | - |
1.8.3.6 | root | ubiquitously expressed in Arabidopsis tissues and organs | Arabidopsis thaliana | - |
1.8.3.6 | seedling | ubiquitously expressed in Arabidopsis tissues and organs | Arabidopsis thaliana | - |
1.8.3.6 | stem | ubiquitously expressed in Arabidopsis tissues and organs | Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.3.5 | N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | Arabidopsis thaliana | (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2 | - |
? | |
1.8.3.5 | N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | Arabidopsis thaliana Col-0 | (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2 | - |
? | |
1.8.3.5 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | Arabidopsis thaliana | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? | |
1.8.3.5 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | Arabidopsis thaliana Col-0 | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? | |
1.8.3.6 | N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | Arabidopsis thaliana | (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2 | - |
? | |
1.8.3.6 | N-acetyl-S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | Arabidopsis thaliana Col-0 | (2E,6E)-farnesal + N-acetyl-L-cysteine + H2O2 | - |
? | |
1.8.3.6 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme is specific for S-farnesyl-L-cysteine. No activity towards S-geranyl-L-cysteine or S-geranylgeranyl-L-cysteine | Arabidopsis thaliana | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? | |
1.8.3.6 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | Arabidopsis thaliana | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? | |
1.8.3.6 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme is specific for S-farnesyl-L-cysteine. No activity towards S-geranyl-L-cysteine or S-geranylgeranyl-L-cysteine | Arabidopsis thaliana Col-0 | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? | |
1.8.3.6 | S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O | the enzyme exhibits specificity for farnesyl-L-cysteine over geranylgeranyl-L-cysteine | Arabidopsis thaliana Col-0 | (2E,6E)-farnesal + L-cysteine + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.3.5 | FC lyase | - |
Arabidopsis thaliana |
1.8.3.5 | FCLY | - |
Arabidopsis thaliana |
1.8.3.6 | At5g63910 | - |
Arabidopsis thaliana |
1.8.3.6 | FC lyase | - |
Arabidopsis thaliana |
1.8.3.6 | FCLY | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.3.5 | 30 | - |
assay at | Arabidopsis thaliana |
1.8.3.6 | 30 | - |
assay at | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.3.5 | 7.5 | - |
assay at | Arabidopsis thaliana |
1.8.3.6 | 7.4 | - |
assay at | Arabidopsis thaliana |
1.8.3.6 | 7.5 | - |
assay at | Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.3.5 | malfunction | T-DNA insertions into the FCLY gene cause significant decreases in FC lyase activity and an enhanced response to abscisic acid in seed germination assays. The effects of FCLY mutations on abscisic acidsensitivity are even greater in the presence of exogenous S-(2E,6E)-farnesyl-L-cysteine | Arabidopsis thaliana |
1.8.3.5 | metabolism | the enzyme is involved in the salvage cycle for S-(2E,6E)-farnesyl diphosphate in plants, overview | Arabidopsis thaliana |
1.8.3.5 | physiological function | Arabidopsis FC lyase recognizes amide-linked S-(2E,6E)-farnesyl-L-cysteine and may have a role in deprenylation of farnesylated proteins | Arabidopsis thaliana |
1.8.3.6 | malfunction | T-DNA insertions into the FCLY gene cause significant decreases in FC lyase activity and an enhanced response to abscisic acid in seed germination assays. The effects of FCLY mutations on abscisic acid sensitivity are even greater in the presence of exogenous farnesyl-L-cysteine | Arabidopsis thaliana |
1.8.3.6 | metabolism | the enzyme is involved in the salvage cycle for S-(2E,6E)-farnesyl diphosphate in plants, overview | Arabidopsis thaliana |
1.8.3.6 | physiological function | Arabidopsis FC lyase recognizes amide-linked S-(2E,6E)-farnesyl-L-cysteine and may have a role in deprenylation of farnesylated proteins | Arabidopsis thaliana |