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Literature summary extracted from

  • Ibdah, M.; Pichersky, E.
    Arabidopsis Chy1 null mutants are deficient in benzoic acid-containing glucosinolates in the seeds (2009), Plant Biol., 11, 574-581.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.4 open-reading frame of the Chy1 gene amplified, cDNA ligated into the vector pEX5-CT/TOPO TA to create a fusion of the open-reading frame with a His tag-coding extension at the C-terminus. Plasmid transferred into Escherichia coli BL21 (DE3) cells for expression Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.4 Cu2+ at 1 mM causes more than 90% inhibition of activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.4 0.0029
-
 cinnamoyl-CoA at 30°C, in 50 mM Tris/HCl buffer, pH 7.0 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.4 additional information Mg2+, Fe2+, Cu2+, Zn2+, and Mn2+ at concentrations of 1 mM have no effect on the hydrolase activity Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.4 Arabidopsis thaliana Q9LKJ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.4 by Ni chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.4 seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.4 3-hydroxy-3-phenylpropionyl-CoA + H2O
-
 Arabidopsis thaliana 3-hydroxy-3-phenylpropionate + CoA
-
 ?
3.1.2.4 cinnamoyl-CoA + H2O
-
 Arabidopsis thaliana cinnamic acid + CoA
-
 ?
3.1.2.4 additional information has no hydrolase activity with benzoyl-CoA Arabidopsis thaliana ?
-
 ?
3.1.2.4 p-coumaroyl-CoA + H2O
-
 Arabidopsis thaliana p-coumaric acid + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.4 CHY1
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.4 7
-
 in 50 mm Tris-buffer Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.1.2.4 malfunction mutations in the Chy1 gene lead to a deficiency of benzoic acid-containing glucosinolates in the seeds Arabidopsis thaliana