EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.2.14 | industry | contribution of FatA to the synthesis of castor oil | Ricinus communis |
3.1.2.14 | industry | contribution of FatB to the synthesis of castor oil | Ricinus communis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.2.14 | PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatA overexpressed in Escherichia coli XL1-Blue | Ricinus communis |
3.1.2.14 | PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatB overexpressed in Escherichia coli XL1-Blue | Ricinus communis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.14 | 0.00035 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.00042 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.00054 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.00054 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.00056 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.00067 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.00086 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.00088 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.2.14 | 36800 | - |
recombinant histidine-tagged FatB, gel filtration | Ricinus communis |
3.1.2.14 | 37520 | - |
recombinant histidine-tagged FatA, gel filtration | Ricinus communis |
3.1.2.14 | 42200 | - |
FatA open reading frames, calculated from sequence | Ricinus communis |
3.1.2.14 | 46500 | - |
FatB open reading frames, calculated from sequence | Ricinus communis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.14 | Ricinus communis | A9XK92 | - |
- |
3.1.2.14 | Ricinus communis | A9XUG5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.14 | to homogeneity in a single step by gel filtration | Ricinus communis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.2.14 | seed | - |
Ricinus communis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.14 | additional information | FatA enzyme displays low levels of activity on saturated stearoyl- and palmitoyl-[acyl-carrier protein] derivatives | Ricinus communis | ? | - |
? | |
3.1.2.14 | oleoyl-[acyl-carrier protein] + H2O | high efficiency of FatB | Ricinus communis | oleic acid + acyl-carrier protein | - |
? | |
3.1.2.14 | oleoyl-[acyl-carrier protein] + H2O | maximum catalytic efficiency of FatA | Ricinus communis | oleic acid + acyl-carrier protein | - |
? | |
3.1.2.14 | palmitoleoyl-[acyl-carrier protein] + H2O | high catalytic efficiency of FatA | Ricinus communis | palmitoleate + acyl-carrier protein | - |
? | |
3.1.2.14 | palmitoleoyl-[acyl-carrier protein] + H2O | high efficiency of FatB | Ricinus communis | palmitoleate + acyl-carrier protein | - |
? | |
3.1.2.14 | palmitoyl-[acyl-carrier protein] + H2O | - |
Ricinus communis | palmitate + acyl-carrier protein | - |
? | |
3.1.2.14 | stearoyl-[acyl-carrier protein] + H2O | - |
Ricinus communis | stearate + acyl-carrier protein | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.2.14 | ? | x * 42200, FatA open reading frames, calculated from sequence | Ricinus communis |
3.1.2.14 | ? | x * 46500, FatA open reading frames, calculated from sequence | Ricinus communis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.2.14 | acyl-ACP thioesterase | - |
Ricinus communis |
3.1.2.14 | acyl-acyl carrier protein (ACP) thioesterase | - |
Ricinus communis |
3.1.2.14 | acylACP thioesterase | - |
Ricinus communis |
3.1.2.14 | acylacyl carrier protein (ACP) thioesterase | - |
Ricinus communis |
3.1.2.14 | FatA | - |
Ricinus communis |
3.1.2.14 | FatB | - |
Ricinus communis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.14 | 0.22 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.38 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.41 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.46 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.56 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 0.67 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 1.62 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 7.99 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.2.14 | Ricinus communis | calculated from sequence | - |
6.7 |
3.1.2.14 | Ricinus communis | calculated from sequence | - |
7 |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.1.2.14 | Ricinus communis | transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation | up |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.14 | 250 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 440 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 760 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 830 | - |
palmitoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 990 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 1030 | - |
stearoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 4630 | - |
palmitoleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis | |
3.1.2.14 | 19030 | - |
oleoyl-[acyl-carrier protein] | in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C | Ricinus communis |