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Literature summary extracted from

  • Sanchez-Garcia, A.; Moreno-Perez, A.J.; Muro-Pastor, A.M.; Salas, J.J.; Garces, R.; Martinez-Force, E.
    Acyl-ACP thioesterases from castor (Ricinus communis L.): An enzymatic system appropriate for high rates of oil synthesis and accumulation (2010), Phytochemistry, 71, 860-869.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.2.14 industry contribution of FatA to the synthesis of castor oil Ricinus communis
3.1.2.14 industry contribution of FatB to the synthesis of castor oil Ricinus communis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatA overexpressed in Escherichia coli XL1-Blue Ricinus communis
3.1.2.14 PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatB overexpressed in Escherichia coli XL1-Blue Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.14 0.00035
-
palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.00042
-
oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.00054
-
palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.00054
-
stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.00056
-
palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.00067
-
oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.00086
-
palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.00088
-
stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.14 36800
-
recombinant histidine-tagged FatB, gel filtration Ricinus communis
3.1.2.14 37520
-
recombinant histidine-tagged FatA, gel filtration Ricinus communis
3.1.2.14 42200
-
FatA open reading frames, calculated from sequence Ricinus communis
3.1.2.14 46500
-
FatB open reading frames, calculated from sequence Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Ricinus communis A9XK92
-
-
3.1.2.14 Ricinus communis A9XUG5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.14 to homogeneity in a single step by gel filtration Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.14 seed
-
Ricinus communis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 additional information FatA enzyme displays low levels of activity on saturated stearoyl- and palmitoyl-[acyl-carrier protein] derivatives Ricinus communis ?
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O high efficiency of FatB Ricinus communis oleic acid + acyl-carrier protein
-
?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O maximum catalytic efficiency of FatA Ricinus communis oleic acid + acyl-carrier protein
-
?
3.1.2.14 palmitoleoyl-[acyl-carrier protein] + H2O high catalytic efficiency of FatA Ricinus communis palmitoleate + acyl-carrier protein
-
?
3.1.2.14 palmitoleoyl-[acyl-carrier protein] + H2O high efficiency of FatB Ricinus communis palmitoleate + acyl-carrier protein
-
?
3.1.2.14 palmitoyl-[acyl-carrier protein] + H2O
-
Ricinus communis palmitate + acyl-carrier protein
-
?
3.1.2.14 stearoyl-[acyl-carrier protein] + H2O
-
Ricinus communis stearate + acyl-carrier protein
-
?

Subunits

EC Number Subunits Comment Organism
3.1.2.14 ? x * 42200, FatA open reading frames, calculated from sequence Ricinus communis
3.1.2.14 ? x * 46500, FatA open reading frames, calculated from sequence Ricinus communis

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 acyl-ACP thioesterase
-
Ricinus communis
3.1.2.14 acyl-acyl carrier protein (ACP) thioesterase
-
Ricinus communis
3.1.2.14 acyl–ACP thioesterase
-
Ricinus communis
3.1.2.14 acyl–acyl carrier protein (ACP) thioesterase
-
Ricinus communis
3.1.2.14 FatA
-
Ricinus communis
3.1.2.14 FatB
-
Ricinus communis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.14 0.22
-
palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.38
-
stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.41
-
palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.46
-
palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.56
-
stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 0.67
-
oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 1.62
-
palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 7.99
-
oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.2.14 Ricinus communis calculated from sequence
-
6.7
3.1.2.14 Ricinus communis calculated from sequence
-
7

Expression

EC Number Organism Comment Expression
3.1.2.14 Ricinus communis transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.14 250
-
palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 440
-
stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 760
-
palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 830
-
palmitoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 990
-
oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 1030
-
stearoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 4630
-
palmitoleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis
3.1.2.14 19030
-
oleoyl-[acyl-carrier protein] in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C Ricinus communis