Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shimada, A.; Masui, R.; Nakagawa, N.; Takahata, Y.; Kim, K.; Kuramitsu, S.; Fukui, K.
    A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways (2010), Nucleic Acids Res., 38, 5692-5705.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.11.1 overexpression in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.11.1 0.13
-
 ssDNA 50 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.16
-
 ssDNA 50 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 0.24
-
 ssDNA 40 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.26
-
 ssDNA 28 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.28
-
 ssDNA 10 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.29
-
 ssDNA 28 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 0.32
-
 ssDNA 40 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 0.35
-
 ssDNA 21 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.44
-
 ssDNA 21 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 0.48
-
 ssDNA 10 mer pH 7.5, 60°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.1 Co2+ activity requires divalent cations Thermus thermophilus
3.1.11.1 Mg2+ activity requires divalent cations Thermus thermophilus
3.1.11.1 Mn2+ activity requires divalent cations Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.1 ssDNA + H2O Thermus thermophilus TTHB178 possesses 3'–5'-ssExo activity that degrades ssDNAs containing deaminated and methylated bases, but not ssDNA containing oxidized bases or abasic sites. The enzyme functions in various DNA repair systems in cooperation with or independently of RecJ ?
-
 ?
3.1.11.1 ssDNA + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579 TTHB178 possesses 3'–5'-ssExo activity that degrades ssDNAs containing deaminated and methylated bases, but not ssDNA containing oxidized bases or abasic sites. The enzyme functions in various DNA repair systems in cooperation with or independently of RecJ ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.1 Thermus thermophilus Q53VZ1
-
-
3.1.11.1 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q53VZ1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.1 additional information no activity against ssRNA and dsDNA is observed Thermus thermophilus ?
-
 ?
3.1.11.1 additional information no activity against ssRNA and dsDNA is observed Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
3.1.11.1 ssDNA + H2O TTHB178 possesses 3'–5'-ssExo activity that degrades ssDNAs containing deaminated and methylated bases, but not ssDNA containing oxidized bases or abasic sites. The enzyme functions in various DNA repair systems in cooperation with or independently of RecJ Thermus thermophilus ?
-
 ?
3.1.11.1 ssDNA + H2O TTHB178 possesses 3'–5'-ssExo activity that degrades ssDNAs containing deaminated and methylated bases, but not ssDNA containing oxidized bases or abasic sites. The enzyme functions in various DNA repair systems in cooperation with or independently of RecJ Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
3.1.11.1 ssDNA 10 mer + H2O
-
 Thermus thermophilus ?
-
 ?
3.1.11.1 ssDNA 10 mer + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
3.1.11.1 ssDNA 21 mer + H2O
-
 Thermus thermophilus ?
-
 ?
3.1.11.1 ssDNA 21 mer + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
3.1.11.1 ssDNA 28 mer + H2O
-
 Thermus thermophilus ?
-
 ?
3.1.11.1 ssDNA 28 mer + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
3.1.11.1 ssDNA 40 mer + H2O
-
 Thermus thermophilus ?
-
 ?
3.1.11.1 ssDNA 50 mer + H2O
-
 Thermus thermophilus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.11.1 30–50 ssExo
-
 Thermus thermophilus
3.1.11.1 single-stranded DNA-specific 3'-5' exonuclease I
-
 Thermus thermophilus
3.1.11.1 tthb178
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.11.1 60
-
 the kcat/KM value is 4-fold to 10-fold higher at 60°C than at 37°C Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.11.1 0.17
-
 ssDNA 40 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.19
-
 ssDNA 50 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.23
-
 ssDNA 28 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.4
-
 ssDNA 21 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.71
-
 ssDNA 10 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 1
-
 ssDNA 50 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 1.9
-
 ssDNA 28 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 2.3
-
 ssDNA 40 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 5.3
-
 ssDNA 21 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 7.5
-
 ssDNA 10 mer pH 7.5, 60°C Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.11.1 0.72
-
 ssDNA 40 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 0.83
-
 ssDNA 28 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 1.1
-
 ssDNA 21 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 1.7
-
 ssDNA 50 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 2.6
-
 ssDNA 10 mer pH 7.5, 37°C Thermus thermophilus
3.1.11.1 6.4
-
 ssDNA 50 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 7
-
 ssDNA 28 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 7.5
-
 ssDNA 40 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 12
-
 ssDNA 21 mer pH 7.5, 60°C Thermus thermophilus
3.1.11.1 16
-
 ssDNA 10 mer pH 7.5, 60°C Thermus thermophilus