Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Brzezicha, B.; Schmidt, M.; Makalowska, I.; Jarmolowski, A.; Pienkowska, J.; Szweykowska-Kulinska, Z.
    Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu(CAA) (2006), Nucleic Acids Res., 34, 6034-6043.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.203 medicine this methyltransferase is a novel downstream Myctarget which mediates Myc-induced cell proliferation and growth. Therefore, the characterization of hTrm4 substrate specificity can be essential since this enzyme is a potential target for cancer therapies Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.202
-
 Saccharomyces cerevisiae
2.1.1.203 expression of the human Trm4 (hTrm4) cDNA in yeast partially complements the lack of the endogenous Trm4p enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.203 nucleolus
-
 Homo sapiens 5730
-
2.1.1.203 nucleoplasm
-
 Homo sapiens 5654
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.203 86471
-
 x * 86471, calculated from sequence Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.203 S-adenosyl-L-methionine + cytosine34 in tRNA Homo sapiens specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA) S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.202 Saccharomyces cerevisiae P38205
-
-
2.1.1.203 Homo sapiens Q08J23
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.203 S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor the formation of 5-methylcytosine34 is a strictly intron-dependent process Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA precursor yeast pre-tRNALeu Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
 ?
2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA yeast tRNASer(AGE) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
-
 ?
2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA yeast tRNAAsp(GUC) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
-
 ?
2.1.1.203 S-adenosyl-L-methionine + cytosine34 in tRNA specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA) Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
 ?
2.1.1.203 S-adenosyl-L-methionine + cytosine34 in tRNA precursor specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA). The hTrm4 MTase has a much narrower specificity against the yeast substrates than its yeast orthologue. The human enzyme is not able to form 5-methylcytosine34 at positions 48 and 49 of human and yeast tRNA precursors. Intron in the human pre-tRNALeu(CAA) is indispensable for the C5-methylation of cytosine in the first position of the anticodon. The modification of C34 depends on the nucleotide sequence surrounding the position to be modified and on the structure of intron-containing prolongated anticodon stem Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.203 ? x * 86471, calculated from sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.1.202 TRM4
-
 Saccharomyces cerevisiae
2.1.1.203 hTrm4
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.202 30
-
 assay at Saccharomyces cerevisiae
2.1.1.203 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.202 7.5
-
 assay at Saccharomyces cerevisiae
2.1.1.203 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.1.1.203 physiological function specific modification of cytosine34 in the intron-containing yeast pre-tRNALeu(CAA) Homo sapiens