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Literature summary extracted from

  • Nakazawa, Y.; Uchino, M.; Sagane, Y.; Sato, H.; Takano, K.
    Isolation and characterization of actinomycetes strains that produce phospholipase D having high transphosphatidylation activity (2009), Microbiol. Res., 164, 43-48.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 additional information Actinobacteria PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, the latter with a primary alcohol, both pathway share a common intermediate, mechanism, overview ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O Actinobacteria
-
 choline + phosphatidic acid
-
 ?
3.1.4.4 phosphatidylethanolamine + H2O Actinobacteria
-
 ethanolamine + phosphatidic acid
-
 ?
3.1.4.4 phosphatidylglycerol + H2O Actinobacteria
-
 glycerol + phosphatidic acid
-
 ?
3.1.4.4 phosphatidylserine + H2O Actinobacteria
-
 serine + phosphatidic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Actinobacteria
-
 diverse strains from soil, isolated from 25 soil samples in Setagaya, Tokyo, Japan
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 additional information
-
 hydrolysis rate of various phospholipids, and conversion rates and selectivity of the various phospholipid-producing-transphosphatidylation reactions of the culture supernatants from selected strains, overview Actinobacteria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 additional information PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, the latter with a primary alcohol, both pathway share a common intermediate, mechanism, overview Actinobacteria ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Actinobacteria choline + phosphatidic acid
-
 ?
3.1.4.4 phosphatidylethanolamine + H2O
-
 Actinobacteria ethanolamine + phosphatidic acid
-
 ?
3.1.4.4 phosphatidylglycerol + H2O
-
 Actinobacteria glycerol + phosphatidic acid
-
 ?
3.1.4.4 phosphatidylserine + H2O
-
 Actinobacteria serine + phosphatidic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 PLD
-
 Actinobacteria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 additional information
-
 transphosphatidylation reaction assay at 37°C Actinobacteria
3.1.4.4 28
-
 in vivo hydrolytic assay at Actinobacteria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 additional information
-
 transphosphatidylation reaction assay at pH 5.5 Actinobacteria