BRENDA - Enzyme Database

Isolation and characterization of actinomycetes strains that produce phospholipase D having high transphosphatidylation activity

Nakazawa, Y.; Uchino, M.; Sagane, Y.; Sato, H.; Takano, K.; Microbiol. Res. 164, 43-48 (2009)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
additional information
Actinobacteria
PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, the latter with a primary alcohol, both pathway share a common intermediate, mechanism, overview
?
-
-
-
3.1.4.4
phosphatidylcholine + H2O
Actinobacteria
-
choline + phosphatidic acid
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
Actinobacteria
-
ethanolamine + phosphatidic acid
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
Actinobacteria
-
glycerol + phosphatidic acid
-
-
?
3.1.4.4
phosphatidylserine + H2O
Actinobacteria
-
serine + phosphatidic acid
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Actinobacteria
-
diverse strains from soil, isolated from 25 soil samples in Setagaya, Tokyo, Japan
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.4
additional information
-
hydrolysis rate of various phospholipids, and conversion rates and selectivity of the various phospholipid-producing-transphosphatidylation reactions of the culture supernatants from selected strains, overview
Actinobacteria
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
additional information
PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, the latter with a primary alcohol, both pathway share a common intermediate, mechanism, overview
709835
Actinobacteria
?
-
-
-
-
3.1.4.4
phosphatidylcholine + H2O
-
709835
Actinobacteria
choline + phosphatidic acid
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
709835
Actinobacteria
ethanolamine + phosphatidic acid
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
-
709835
Actinobacteria
glycerol + phosphatidic acid
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
-
709835
Actinobacteria
serine + phosphatidic acid
-
-
-
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.1.4.4
additional information
-
transphosphatidylation reaction assay at 37░C
Actinobacteria
3.1.4.4
28
-
in vivo hydrolytic assay at
Actinobacteria
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
additional information
-
transphosphatidylation reaction assay at pH 5.5
Actinobacteria
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
additional information
Actinobacteria
PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, the latter with a primary alcohol, both pathway share a common intermediate, mechanism, overview
?
-
-
-
3.1.4.4
phosphatidylcholine + H2O
Actinobacteria
-
choline + phosphatidic acid
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
Actinobacteria
-
ethanolamine + phosphatidic acid
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
Actinobacteria
-
glycerol + phosphatidic acid
-
-
?
3.1.4.4
phosphatidylserine + H2O
Actinobacteria
-
serine + phosphatidic acid
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.4
additional information
-
hydrolysis rate of various phospholipids, and conversion rates and selectivity of the various phospholipid-producing-transphosphatidylation reactions of the culture supernatants from selected strains, overview
Actinobacteria
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
additional information
PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, the latter with a primary alcohol, both pathway share a common intermediate, mechanism, overview
709835
Actinobacteria
?
-
-
-
-
3.1.4.4
phosphatidylcholine + H2O
-
709835
Actinobacteria
choline + phosphatidic acid
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
709835
Actinobacteria
ethanolamine + phosphatidic acid
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
-
709835
Actinobacteria
glycerol + phosphatidic acid
-
-
-
?
3.1.4.4
phosphatidylserine + H2O
-
709835
Actinobacteria
serine + phosphatidic acid
-
-
-
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.1.4.4
additional information
-
transphosphatidylation reaction assay at 37░C
Actinobacteria
3.1.4.4
28
-
in vivo hydrolytic assay at
Actinobacteria
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
additional information
-
transphosphatidylation reaction assay at pH 5.5
Actinobacteria