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Literature summary extracted from

  • Peng, Y.; Liu, G.L.; Yu, X.J.; Wang, X.H.; Jing, L.; Chi, Z.M.
    Cloning of exo-beta-1,3-glucanase gene from a marine yeast Williopsis saturnus and its overexpression in Yarrowia lipolytica (2011), Mar. Biotechnol., 13, 193-204.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.58 gene WsEXG1, cloned from from both the genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, subcloning of a His6-tagged enzyme in Escherichia coli strain DH5alpha, functional overexpression in Yarrowia lipolytica strain Po1h Cyberlindnera saturnus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 1,10-phenanthroline 40.5% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Ag+ over 97% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Co2+ 76.5% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Cu2+ 87.7% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 EDTA 99% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 EGTA 94.5% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Fe2+ 18.8% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Fe3+ over 97% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Hg2+ over 97% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 iodoacetic acid 74.4% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Mn2+ 87.5% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Ni2+ 83.5% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 PMSF 24% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 SDS 34% inhibition at 5 mM Cyberlindnera saturnus
3.2.1.58 Zn2+ 76% inhibition at 5 mM Cyberlindnera saturnus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.58 Ba2+ activates 12% at 5 mM Cyberlindnera saturnus
3.2.1.58 additional information no effect by 5 mM of Mg2+, Ca2+, Na+, K+, or Li+ Cyberlindnera saturnus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 46000
-
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE Cyberlindnera saturnus
3.2.1.58 46200
-
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE Cyberlindnera saturnus
3.2.1.58 47500
-
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE Cyberlindnera saturnus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Cyberlindnera saturnus C3VMY4 i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1
-
3.2.1.58 no activity in Yarrowia lipolytica
-
-
-
3.2.1.58 no activity in Yarrowia lipolytica Po1h
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58 recombinant His6-tagged enzyme 2.1fold from Yarrowia lipolytica strain Po1h to homogeneity Cyberlindnera saturnus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 62
-
crude enzyme from wild-type strain WC91-2 Cyberlindnera saturnus
3.2.1.58 249
-
crude recombinant enzyme from overexpressing Yarrowia lipolytica strain Po1h Cyberlindnera saturnus
3.2.1.58 511
-
purified recombinant enzyme Cyberlindnera saturnus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 laminarin + H2O active site glutamate residue Cyberlindnera saturnus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE Cyberlindnera saturnus
3.2.1.58 More enzyme model with active site glutamate residue, overview Cyberlindnera saturnus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.58 exo-beta-1,3-glucanase
-
Cyberlindnera saturnus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 40
-
-
Cyberlindnera saturnus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.58 30 55 activity range Cyberlindnera saturnus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.58 40
-
purified recombinant enzyme, stable up to Cyberlindnera saturnus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 5
-
-
Cyberlindnera saturnus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.58 3 9.5 recombinant enzyme, 4°C, 2 h, stable Cyberlindnera saturnus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.58 Cyberlindnera saturnus sequence calculation
-
4.5