BRENDA - Enzyme Database

The tumor suppressor gene H-Rev107 functions as a novel Ca2+-independent cytosolic phospholipase A1/2 of the thiol hydrolase type

Uyama, T.; Morishita, J.; Jin, X.H.; Okamoto, Y.; Tsuboi, K.; Ueda, N.; J. Lipid Res. 50, 685-693 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.32
dithiothreitol
-
Homo sapiens
3.1.1.32
dithiothreitol
-
Mus musculus
3.1.1.32
dithiothreitol
required for full activity. Addition of 2 mM increases PLA1/2 activity 167fold
Rattus norvegicus
3.1.1.32
additional information
purified H-Rev107 exhibits an 88fold higher PLA1/2 activity than the cytosolic fraction
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.32
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
Homo sapiens
3.1.1.32
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
Mus musculus
3.1.1.32
expression vector harboring H-Rev107 with a FLAG tag at the N terminus. Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
Rattus norvegicus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.32
additional information
deletion mutant of H-Rev107 lacking either N-terminal 8 or N-terminal 16 amino acids (DELTAN1, DELTAN2) or C-terminal 25 amino acids (DELTAC1). Expression of the mutants with the expected molecular masses. As for PLA1/2 activity, DELTAN1 shows very low activity (about 3% that of the wild-type), and DELTAN2 and DELTAC1 are entirely inactive
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
iodoacetic acid
-
Homo sapiens
3.1.1.32
iodoacetic acid
-
Mus musculus
3.1.1.32
iodoacetic acid
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
cytosol
-
Homo sapiens
5829
-
3.1.1.32
cytosol
-
Mus musculus
5829
-
3.1.1.32
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
additional information
H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2
Homo sapiens
3.1.1.32
additional information
H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2
Mus musculus
3.1.1.32
additional information
H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.32
19000
-
x * 19000, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Homo sapiens
-
-
-
3.1.1.32
Mus musculus
-
-
-
3.1.1.32
Rattus norvegicus
P53817
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.32
-
Homo sapiens
3.1.1.32
-
Mus musculus
3.1.1.32
FLAG-tagged H-Rev107 by immunoprecipitation
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
brain
-
Mus musculus
-
3.1.1.32
testis
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
dipalmitoylphosphatidylcholine + H2O
-
709425
Mus musculus
palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
-
-
?
3.1.1.32
dipalmitoylphosphatidylcholine + H2O
-
709425
Homo sapiens
palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
-
-
?
3.1.1.32
dipalmitoylphosphatidylcholine + H2O
-
709425
Rattus norvegicus
palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
-
-
?
3.1.1.32
additional information
His21 and Cys111 of H-Rev107 form a catalytic dyad. The N-terminal proline-rich and C-terminal hydrophobic domains are involved in the catalytic activity and/or proper folding
709425
Rattus norvegicus
?
-
-
-
-
3.1.1.32
phosphatidylcholine + H2O
-
709425
Mus musculus
2-acylglycerophosphocholine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylcholine + H2O
-
709425
Homo sapiens
2-acylglycerophosphocholine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylcholine + H2O
-
709425
Rattus norvegicus
2-acylglycerophosphocholine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
709425
Mus musculus
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
709425
Homo sapiens
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
709425
Rattus norvegicus
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.32
?
x * 19000, SDS-PAGE
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.32
dithiothreitol
-
Homo sapiens
3.1.1.32
dithiothreitol
-
Mus musculus
3.1.1.32
dithiothreitol
required for full activity. Addition of 2 mM increases PLA1/2 activity 167fold
Rattus norvegicus
3.1.1.32
additional information
purified H-Rev107 exhibits an 88fold higher PLA1/2 activity than the cytosolic fraction
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.32
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
Homo sapiens
3.1.1.32
cDNA encoding C-terminally FLAG-tagged H-Rev107. Subcloned into the HindIII and EcoRI sites of pcDNA3.1 (+). Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
Mus musculus
3.1.1.32
expression vector harboring H-Rev107 with a FLAG tag at the N terminus. Expression vector harboring H-Rev107 cDNA introduced into COS-7 cells
Rattus norvegicus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.32
additional information
deletion mutant of H-Rev107 lacking either N-terminal 8 or N-terminal 16 amino acids (DELTAN1, DELTAN2) or C-terminal 25 amino acids (DELTAC1). Expression of the mutants with the expected molecular masses. As for PLA1/2 activity, DELTAN1 shows very low activity (about 3% that of the wild-type), and DELTAN2 and DELTAC1 are entirely inactive
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
iodoacetic acid
-
Homo sapiens
3.1.1.32
iodoacetic acid
-
Mus musculus
3.1.1.32
iodoacetic acid
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
cytosol
-
Homo sapiens
5829
-
3.1.1.32
cytosol
-
Mus musculus
5829
-
3.1.1.32
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
additional information
H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2
Homo sapiens
3.1.1.32
additional information
H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2
Mus musculus
3.1.1.32
additional information
H-Rev107 functions as a cytosolic Ca2+-independent PLA1/2
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.32
19000
-
x * 19000, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.32
-
Homo sapiens
3.1.1.32
-
Mus musculus
3.1.1.32
FLAG-tagged H-Rev107 by immunoprecipitation
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
brain
-
Mus musculus
-
3.1.1.32
testis
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
dipalmitoylphosphatidylcholine + H2O
-
709425
Mus musculus
palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
-
-
?
3.1.1.32
dipalmitoylphosphatidylcholine + H2O
-
709425
Homo sapiens
palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
-
-
?
3.1.1.32
dipalmitoylphosphatidylcholine + H2O
-
709425
Rattus norvegicus
palmitic acid + 1-lyso-2-palmitoylphosphatidylcholine
-
-
-
?
3.1.1.32
additional information
His21 and Cys111 of H-Rev107 form a catalytic dyad. The N-terminal proline-rich and C-terminal hydrophobic domains are involved in the catalytic activity and/or proper folding
709425
Rattus norvegicus
?
-
-
-
-
3.1.1.32
phosphatidylcholine + H2O
-
709425
Mus musculus
2-acylglycerophosphocholine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylcholine + H2O
-
709425
Homo sapiens
2-acylglycerophosphocholine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylcholine + H2O
-
709425
Rattus norvegicus
2-acylglycerophosphocholine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
709425
Mus musculus
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
709425
Homo sapiens
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
709425
Rattus norvegicus
2-acylglycerophosphoethanolamine + a carboxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.32
?
x * 19000, SDS-PAGE
Rattus norvegicus
General Information
EC Number
General Information
Commentary
Organism
3.1.1.32
physiological function
H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation
Homo sapiens
3.1.1.32
physiological function
H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation
Mus musculus
3.1.1.32
physiological function
H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation
Rattus norvegicus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.1.32
physiological function
H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation
Homo sapiens
3.1.1.32
physiological function
H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation
Mus musculus
3.1.1.32
physiological function
H-Rev107 functions principally as a Ca2+-independent PLA1/2 with a higher PLA1 activity. Transient expression of H-Rev107 in COS-7 cells does not appear to influence cell proliferation
Rattus norvegicus