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Literature summary extracted from

  • Campos-Bermudez, V.A.; Moran-Barrio, J.; Costa-Filho, A.J.; Vila, A.J.
    Metal-dependent inhibition of glyoxalase II: A possible mechanism to regulate the enzyme activity (2010), J. Inorg. Biochem., 104, 726-731.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.6 pET32-gloB vector overexpressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 glutathione metal-selective end product inhibition. Thiol-Fe bond is formed between the Cys moiety of glutathione and the metal site, the dinuclear iron sites show an enhanced antiferromagnetic coupling in the GloB-glutathione adduct Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.6 Fe2+ behavior of Fe-GloB resembles that of Zn-GloB. Thiol-Fe bond is formed between the Cys moiety of glutathione and the metal site, the dinuclear iron sites show an enhanced antiferromagnetic coupling in the GloB-glutathione adduct. GloB-glutathione complex displays S-thiol-FeIII and S-thiol-FeII: charge transfer bands Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 Fe3+ GloB-glutathione complex displays S-thiol-FeIII and S-thiol-FeII: charge transfer bands Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 Mn2+ MnII-thiolate bonds are less covalent and weaker than iron–thiolate bonds Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 Zn2+ behavior of Zn-GloB resembles that of Fe-GloB Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
3.1.2.6 Salmonella enterica subsp. enterica serovar Typhimurium 14028s
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.6 iron-enriched GloB, manganese-enriched GloB and zinc-enriched GloB Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 S-D-lactoylglutathione + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium D-lactate + glutathione
-
 ?
3.1.2.6 S-D-lactoylglutathione + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 14028s D-lactate + glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 GloB
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 GLX2
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 glyoxalase II
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.2.6 hydroxyacylglutathione hydrolase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.6 170
-
 S-D-lactoylglutathione in 10 mM MOPS buffer at pH 7.2, at 25 °C Salmonella enterica subsp. enterica serovar Typhimurium

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.2.6 additional information
-
 Fe- and Zn-GloB is inhibited at submillimolar concentrations of glutathione, in 10 mM MOPS buffer, 0.2 M NaCl, pH 7.2, at 30 °C Salmonella enterica subsp. enterica serovar Typhimurium additional information
3.1.2.6 1.5
-
 Mn-GloB, in 10 mM MOPS buffer, 0.2 M NaCl, pH 7.2, at 30 °C Salmonella enterica subsp. enterica serovar Typhimurium glutathione

General Information

EC Number General Information Comment Organism
3.1.2.6 physiological function assembly of a specific metal-enriched form of GLX2 may be a mechanism to regulate the enzyme activity in vivo Salmonella enterica subsp. enterica serovar Typhimurium