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Literature summary extracted from

  • Chatterjee, S.; Lardinois, O.; Bonini, M.G.; Bhattacharjee, S.; Stadler, K.; Corbett, J.; Deterding, L.J.; Tomer, K.B.; Kadiiska, M.; Mason, R.P.
    Site-specific carboxypeptidase B1 tyrosine nitration and pathophysiological implications following its physical association with nitric oxide synthase-3 in experimental sepsis (2009), J. Immunol., 183, 4055-4066.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.17.2 cytosol confocal microscopy results indicate that levels of both CPB1 and nitric oxide synthase-3 are high in the septic spleen and that CPB1 colocalizes with nitric oxide synthase-3 in the sinus-lining cells of the red pulp. LPS treatment results in the translocation of nitric oxide synthase-3 to the cytosol and colocalization with CPB1 Mus musculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.2 32000
-
 Western blot analysis, investigation of peroxynitrite-induced chemical nitrosylation of CPB Sus scrofa
3.4.17.2 35000
-
 Western blot analysis, investigation of peroxynitrite-induced chemical nitrosylation of CPB Sus scrofa
3.4.17.2 47000
-
 Western Blot analysis, nitrosylated CPB1 Mus musculus
3.4.17.2 70000
-
 Western blot analysis, investigation of peroxynitrite-induced chemical nitrosylation of CPB, dimer formation, band immunoreactive to an antibody specific to 3-nitrotyrosine Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.2 Mus musculus
-
-
-
3.4.17.2 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.17.2 nitrosylation LPS-induced septic shock produces tyrosine nitration in vivo which is due to the formation of peroxynitrite and is mediated by xanthine oxidase and nitric oxide synthase-3. Nitrosylation leads to the inactivation of the enzyme Mus musculus
3.4.17.2 nitrosylation peroxynitrite-dependent tyrosine nitration leading to inactivation of the enzyme. At least 11 peptides with five sites of nitration on tyrosine residues are identified. These include Tyr92, Tyr210, Tyr277, Tyr248, and Tyr198. Of these, Tyr248 and Tyr198 are located in the catalytic site of CPB Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.2 spleen confocal microscopy results indicate that levels of both CPB1 and nitric oxide synthase-3 are high in the septic spleen and that CPB1 colocalizes with nitric oxide synthase-3 in the sinus-lining cells of the red pulp. LPS treatment results in the translocation of nitric oxide synthase-3 to the cytosol and colocalization with CPB1 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.2 Hippuryl-L-Arg + H2O
-
 Mus musculus Hippuric acid + L-Arg
-
 ?
3.4.17.2 Hippuryl-L-Arg + H2O
-
 Sus scrofa Hippuric acid + L-Arg
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.17.2 dimer 2 * 35000, SDS-PAGE, dimer formation as a result of dityrosine crosslinks Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.4.17.2 carboxypeptidase B
-
 Sus scrofa
3.4.17.2 Carboxypeptidase B1
-
 Mus musculus
3.4.17.2 CPB
-
 Sus scrofa
3.4.17.2 CPB1
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.2 25
-
 assay at Mus musculus
3.4.17.2 25
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.2 7.65
-
 assay at Mus musculus
3.4.17.2 7.65
-
 assay at Sus scrofa