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Literature summary extracted from

  • Laslo, A.C.; Ganea, E.; Obinger, C.
    Refolding of hexameric porcine leucine aminopeptidase using a cationic detergent and dextrin-10 as artificial chaperones (2009), J. Biotechnol., 140, 162-168.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.11.1 hexameric LAP is almost completely inactivated, showing 0.6% residual activity, by incubation with 2.5 M GuHCl and 40 mM DTT for 3 h at room temperature Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 guanidinium hydrochloride hexameric LAP is almost completely inactivated, showing 0.6% residual activity, by incubation with 2.5 M GuHCl and 40 mM DTT for 3 h at room temperature Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 14.2
-
 L-leucylamide native enzyme, pH 8.0, 25°C Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Sus scrofa
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.11.1 guanidinium hydrochloride-denatured hexameric leucine aminopeptidase is efficiently refolded by using the cationic detergent cetyltrimethylammonium bromide and the linear polysaccharide dextrin-10 as artificial chaperones leading to 92% of fully active and correct folded LAP, method and kinetics, overview. Recovery of the enzymatic activity using methyl-beta-cyclodextrin was calculated to be 37%, 55%, 64% and 72% after 1 h, 5 h, 10 h and 20 h of incubation, respectively Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 commercial preparation
-
 Sus scrofa
-
3.4.11.1 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-leucylamide + H2O
-
 Sus scrofa L-leucine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 hexamer oligomeric structure, overview Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 leucine aminopeptidase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 25
-
 assay at Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 112
-
 L-leucylamide native enzyme, pH 8.0, 25°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
 assay at Sus scrofa