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Literature summary extracted from

  • Watanabe, T.; Ikeda, H.; Masaki, K.; Fujii, T.; Iefuji, H.
    Cloning and characterization of a novel phytase from wastewater treatment yeast Hansenula fabianii J640 and expression in Pichia pastoris (2009), J. Biosci. Bioeng., 108, 225-230.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.26 Zn2+ 122% of initial activity Cyberlindnera fabianii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.26 expression in Pichia pastoris Cyberlindnera fabianii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 51000
-
 x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE Cyberlindnera fabianii
3.1.3.26 75000
-
 x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE Cyberlindnera fabianii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Cyberlindnera fabianii C4B6T1
-
-
3.1.3.26 Cyberlindnera fabianii J640 C4B6T1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.26 glycoprotein
-
 Cyberlindnera fabianii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 10.2
-
 substrate D-glucose 1-phosphate, 37°C, pH 4.5 Cyberlindnera fabianii
3.1.3.26 22
-
 substrate alpha-glycerophosphate, 37°C, pH 4.5 Cyberlindnera fabianii
3.1.3.26 31.6
-
 substrate beta-glycerophosphate, 37°C, pH 4.5 Cyberlindnera fabianii
3.1.3.26 31.7
-
 substrate D-glucose 6-phosphate, 37°C, pH 4.5 Cyberlindnera fabianii
3.1.3.26 46.1
-
 substrate myo-inositol hexakisphosphate, 37°C, pH 4.5 Cyberlindnera fabianii
3.1.3.26 185
-
 substrate 4-nitrophenyl phosphate, 37°C, pH 4.5 Cyberlindnera fabianii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 4-nitrophenyl phosphate + H2O
-
 Cyberlindnera fabianii 4-nitrophenol + phosphate
-
 ?
3.1.3.26 4-nitrophenyl phosphate + H2O
-
 Cyberlindnera fabianii J640 4-nitrophenol + phosphate
-
 ?
3.1.3.26 alpha-glycerophosphate + H2O
-
 Cyberlindnera fabianii glycerol + phosphate
-
 ?
3.1.3.26 alpha-glycerophosphate + H2O
-
 Cyberlindnera fabianii J640 glycerol + phosphate
-
 ?
3.1.3.26 beta-glycerophosphate + H2O
-
 Cyberlindnera fabianii glycerol + phosphate
-
 ?
3.1.3.26 beta-glycerophosphate + H2O
-
 Cyberlindnera fabianii J640 glycerol + phosphate
-
 ?
3.1.3.26 D-glucose 1-phosphate + H2O
-
 Cyberlindnera fabianii D-glucose + phosphate
-
 ?
3.1.3.26 D-glucose 6-phosphate + H2O
-
 Cyberlindnera fabianii D-glucose + phosphate
-
 ?
3.1.3.26 D-glucose 6-phosphate + H2O
-
 Cyberlindnera fabianii J640 D-glucose + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Cyberlindnera fabianii ? + phosphate removal of 5 phosphate residues from each molecule of phytate ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Cyberlindnera fabianii J640 ? + phosphate removal of 5 phosphate residues from each molecule of phytate ?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 ? x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE Cyberlindnera fabianii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 50
-
-
 Cyberlindnera fabianii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 4.5
-
-
 Cyberlindnera fabianii