Any feedback?
Literature summary extracted from
- Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin: Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes (2000), J. Biol. Chem., 275, 1966-1975.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.38 | expression in Pichia pastoris | Manihot esculenta |
| 1.14.14.39 | expression in Pichia pastoris | Manihot esculenta |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.38 | diphenyleneiodonium chloride | - |
Manihot esculenta |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.38 | 1.3 | - |
L-isoleucine | pH 7.9, 30°C | Manihot esculenta | |
| 1.14.14.38 | 2.2 | - |
L-valine | pH 7.9, 30°C | Manihot esculenta | |
| 1.14.14.39 | 1.3 | - |
L-isoleucine | pH 7.9, 30°C | Manihot esculenta | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.38 | microsome | - |
Manihot esculenta | - |
- |
| 1.14.14.39 | microsome | - |
Manihot esculenta | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.38 | 61200 | - |
- |
Manihot esculenta |
| 1.14.14.38 | 62000 | - |
x * 62000, SDS-PAGE and calculated | Manihot esculenta |
| 1.14.14.39 | 61200 | - |
- |
Manihot esculenta |
| 1.14.14.39 | 62000 | - |
- |
Manihot esculenta |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.38 | Manihot esculenta | Q9M7B7 | - |
- |
| 1.14.14.38 | Manihot esculenta | Q9M7B8 | - |
- |
| 1.14.14.39 | Manihot esculenta | Q9M7B7 | cf. EC 1.14.14.38 | - |
| 1.14.14.39 | Manihot esculenta | Q9M7B8 | cf. EC 1.14.14.38 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.14.14.38 | glycoprotein | glycosylation of the asparagine residues at the N-terminus | Manihot esculenta |
| 1.14.14.38 | glycoprotein | recombinant protein expressed in Pichia pastoris is glycosylated | Manihot esculenta |
| 1.14.14.39 | glycoprotein | recombinant protein expressed in Pichia pastoris is glycosylated | Manihot esculenta |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.38 | recombinant enzyme, reconstitution in lipid micelles | Manihot esculenta |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.38 | leaf | - |
Manihot esculenta | - |
| 1.14.14.39 | leaf | - |
Manihot esculenta | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.38 | L-isoleucine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Manihot esculenta | (1E,2S)-2-methylbutanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
| 1.14.14.38 | L-isoleucine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | under saturating substrate conditions CYP79D1 has a higher conversion rate using L-valine as substrate. The conversion rate of L-isoleucine is approximately 60% of that observed for L-valine, consistent with higher accumulation of linamarin compared with lotaustralin in vivo in cassava | Manihot esculenta | (1E,2S)-2-methylbutanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
| 1.14.14.38 | L-valine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Manihot esculenta | (E)-2-methylpropanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | overall reaction | ? | |
| 1.14.14.38 | L-valine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Manihot esculenta | (E)-2-methylpropanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
| 1.14.14.38 | L-valine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | under saturating substrate conditions CYP79D1 has a higher conversion rate using L-valine as substrate. The conversion rate of L-isoleucine is approximately 60% of that observed for L-valine, consistent with higher accumulation of linamarin compared with lotaustralin in vivo in cassava | Manihot esculenta | (E)-2-methylpropanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
| 1.14.14.38 | additional information | no substrate: L-leucine, L-phenylalanine, L-tyrosine. The observed substrate specificity corresponds with the in vivo presence of only L-valine- and L-isoleucine-derived cyanogenic glucosides in cassava | Manihot esculenta | ? | - |
? | |
| 1.14.14.38 | additional information | enzyme additionally acts on L-isoleucine, reaction of EC 1.14.14.39. The conversion rate of L-isoleucine is approximately 60% of that observed for L-valine. No substrates: D-valine, D-isoleucine, L-leucine, L-phenylalanine, or L-tyrosine | Manihot esculenta | ? | - |
? | |
| 1.14.14.38 | additional information | enzyme additionally acts on L-valine, reaction of EC 1.14.14.38. No substrates: D-valine, D-isoleucine, L-leucine, L-phenylalanine, or L-tyrosine | Manihot esculenta | ? | - |
? | |
| 1.14.14.39 | L-isoleucine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Manihot esculenta | (1E,2S)-2-methylbutanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | overall reaction | ? | |
| 1.14.14.39 | additional information | enzyme additionally acts on L-valine, reaction of EC 1.14.14.38. No substrates: D-valine, D-isoleucine, L-leucine, L-phenylalanine, or L-tyrosine | Manihot esculenta | ? | - |
? | |
| 1.14.14.39 | additional information | enzyme additionally acts on L-valine, reaction of EC 1.14.14.38. The conversion rate of L-isoleucine is approximately 60% of that observed for L-valine. No substrates: D-valine, D-isoleucine, L-leucine, L-phenylalanine, or L-tyrosine | Manihot esculenta | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.38 | ? | x * 62000, SDS-PAGE and calculated | Manihot esculenta |
| 1.14.14.38 | ? | x * 61200, calculated, x * 62000, SDS-PAGE | Manihot esculenta |
| 1.14.14.39 | ? | x * 61200, calculated, x * 62000, SDS-PAGE | Manihot esculenta |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.38 | CYP79D1 | - |
Manihot esculenta |
| 1.14.14.38 | CYP79D2 | - |
Manihot esculenta |
| 1.14.14.38 | N-hydroxylating cytochrome P450 | - |
Manihot esculenta |
| 1.14.14.38 | valine N-monooxygenase | - |
Manihot esculenta |
| 1.14.14.39 | CYP79D1 | - |
Manihot esculenta |
| 1.14.14.39 | CYP79D2 | - |
Manihot esculenta |
| 1.14.14.39 | valine N-monooxygenase | - |
Manihot esculenta |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.38 | 30 | - |
assay at | Manihot esculenta |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.38 | 0.103 | - |
L-isoleucine | pH 7.9, 30°C | Manihot esculenta | |
| 1.14.14.38 | 0.16 | - |
L-valine | pH 7.9, 30°C | Manihot esculenta | |
| 1.14.14.38 | 0.162 | - |
L-valine | pH 7.9, 30°C | Manihot esculenta | |
| 1.14.14.39 | 0.1 | - |
L-isoleucine | pH 7.9, 30°C | Manihot esculenta | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.38 | 7.9 | - |
assay at | Manihot esculenta |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.38 | physiological function | bifunctional enzyme, metabolizes L-valine as well as L-isoleucine, i.e. activities of EC 1.14.14.38 and 1.14.14.39, consistent with the cooccurrence of linamarin and lotaustralin in cassava | Manihot esculenta |
| 1.14.14.38 | physiological function | bifunctional enzyme, metabolizes L-valine as well as L-isoleucine, i.e. activities of EC 1.14.14.38 and 1.14.14.39, consistent with the cooccurrence of linamarin and lotaustralin in cassava. CYP79D1 has a higher kcat value with L-valine as substrate than with L-isoleucine, which is consistent with linamarin being the major cyanogenic glucoside in cassava | Manihot esculenta |
| 1.14.14.39 | physiological function | bifunctional enzyme, metabolizes L-valine as well as L-isoleucine, i.e. activities of EC 1.14.14.38 and 1.14.14.39, consistent with the cooccurrence of linamarin and lotaustralin in cassava | Manihot esculenta |
| 1.14.14.39 | physiological function | bifunctional enzyme, metabolizes L-valine as well as L-isoleucine, i.e. activities of EC 1.14.14.38 and 1.14.14.39, consistent with the cooccurrence of linamarin and lotaustralin in cassava. CYP79D1 has a higher kcat value with L-valine as substrate than with L-isoleucine, which is consistent with linamarin being the major cyanogenic glucoside in cassava | Manihot esculenta |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.38 | 0.074 | - |
L-valine | pH 7.9, 30°C | Manihot esculenta | |
| 1.14.14.38 | 0.1 | - |
L-isoleucine | pH 7.9, 30°C | Manihot esculenta | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
