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Literature summary extracted from

  • Wickwire, B.M.; Wagner, C.; Broquist, H.P.
    Pipecolic acid biosynthesis in Rhizoctonia leguminicola. II. Saccharopine oxidase: a unique flavin enzyme involved in pipecolic acid biosynthesis (1990), J. Biol. Chem., 265, 14748-14753.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.18 45000
-
 gel filtration Slafractonia leguminicola
1.5.3.18 52000
-
 1 * 52000, SDS-PAGE Slafractonia leguminicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.18 L-saccharopine + H2O + O2 Slafractonia leguminicola the enzyme shunts saccharopine, a major lysine metabolite, into a secondary pathway of lysine metabolism leading to pipecolate and subsequently to slaframine and swainsonine L-2-aminoadipate 6-semialdehyde + L-glutamate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.18 Slafractonia leguminicola
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.18
-
 Slafractonia leguminicola

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.18 mycelium
-
 Slafractonia leguminicola
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.18 L-saccharopine + H2O + O2
-
 Slafractonia leguminicola L-2-aminoadipate 6-semialdehyde + L-glutamate + H2O2
-
 ?
1.5.3.18 L-saccharopine + H2O + O2 the enzyme shunts saccharopine, a major lysine metabolite, into a secondary pathway of lysine metabolism leading to pipecolate and subsequently to slaframine and swainsonine Slafractonia leguminicola L-2-aminoadipate 6-semialdehyde + L-glutamate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.18 monomer 1 * 52000, SDS-PAGE Slafractonia leguminicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.18 37
-
 assay at Slafractonia leguminicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.18 6
-
-
 Slafractonia leguminicola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.18 5 7.5 pH 5.0: about 45% of maximal activity at pH 5.0 and pH 7.5 Slafractonia leguminicola

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.18 flavin presence of a flavin prosthetic group Slafractonia leguminicola

General Information

EC Number General Information Comment Organism
1.5.3.18 physiological function involved in pipecolic acid biosynthesis Slafractonia leguminicola