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Literature summary extracted from

  • Bralley, P.; Cozad, M.; Jones, G.
    Geobacter sulfurreducens contains separate C- and A-adding tRNA nucleotidyltransferases and a poly(A) polymerase (2009), J. Bacteriol., 91, 109-114.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.72 expressed in Escherichia coli Alkalihalobacillus clausii
2.7.7.72 expressed in Escherichia coli Thermus thermophilus
2.7.7.72 expressed in Escherichia coli BL21(DE3)/pLysS Rosetta cells Geobacter sulfurreducens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.72 45590
-
 NTSFII Geobacter sulfurreducens
2.7.7.72 98390
-
 NTSFIII Geobacter sulfurreducens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.72 Alkalihalobacillus clausii Q5WGA1
-
-
2.7.7.72 Geobacter sulfurreducens
-
 strain ATCC 51573
-
2.7.7.72 Thermus thermophilus Q72K91
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.72 purified by a combination of ammonium sulfate fractionation, gel filtration, and hydrophobic interaction chromatography (NTSFII) Geobacter sulfurreducens
2.7.7.72 purified by a combination of anion-exchange chromatography and hydrophobic interaction chromatography Geobacter sulfurreducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.72 ATP + tRNA-C-C
-
 Geobacter sulfurreducens tRNA-C-C-A + diphosphate
-
 ?
2.7.7.72 ATP + tRNA-C-C
-
 Alkalihalobacillus clausii tRNA-C-C-A + diphosphate
-
 ?
2.7.7.72 ATP + tRNA-C-C
-
 Thermus thermophilus tRNA-C-C-A + diphosphate
-
 ?
2.7.7.72 CTP + tRNA-C
-
 Geobacter sulfurreducens tRNA-C-C + diphosphate
-
 ?
2.7.7.72 additional information NTSFIII is inactive with CTP and tRNAAsp-C as substrates Geobacter sulfurreducens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.72 A-adding tRNA nucleotidyltransferase
-
 Geobacter sulfurreducens
2.7.7.72 C-adding tRNA nucleotidyltransferase
-
 Geobacter sulfurreducens
2.7.7.72 CCA-adding enzyme
-
 Alkalihalobacillus clausii
2.7.7.72 CCA-adding enzyme
-
 Thermus thermophilus
2.7.7.72 CCA-adding enzyme/poly(A) polymerase
-
 Alkalihalobacillus clausii
2.7.7.72 CCA-adding enzyme/poly(A) polymerase
-
 Thermus thermophilus
2.7.7.72 NTSFII
-
 Geobacter sulfurreducens
2.7.7.72 NTSFIII
-
 Geobacter sulfurreducens
2.7.7.72 TNT
-
 Geobacter sulfurreducens