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Literature summary extracted from

  • Yin, L.J.; Lin, H.H.; Chiang, Y.I.; Jiang, S.T.
    Bioproperties and purification of xylanase from Bacillus sp. YJ6 (2010), J. Agric. Food Chem., 58, 557-562.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2-mercaptoethanol native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 glutathione native enzyme Bacillus sp. (in: Bacteria)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 DNA and amino acid sequence determination and analysis, expression in Escherichia coli Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+ inhibits the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 Fe3+ inhibits the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 Hg2+ inhibits the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 leupeptin inhibits the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 N-ethylmaleimide inhibits the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 N-tosyl-L-phenylalanine chloromethyl ketone inhibits the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 PMSF inhibits the native enzyme Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Co2+ activates the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 K+ activates the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 Mg2+ activates the native enzyme Bacillus sp. (in: Bacteria)
3.2.1.8 Na+ activates the native enzyme Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 19000
-
 x * 19000, native enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O Bacillus sp. (in: Bacteria) YJ6
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.8 Bacillus sp. (in: Bacteria) YJ6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 native enzyme 678.1fold from strain YJ6 to homogeneity by ammonium sulfate fractionation, dialysis, cation exchange chromatography, ultrafiltration, and gel filtration Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 1436
-
 purified native enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O
-
 Bacillus sp. (in: Bacteria) YJ6 ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood Bacillus sp. (in: Bacteria) YJ6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 19000, native enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 purified native enzyme, stable Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5 9 purified native enzyme, stable Bacillus sp. (in: Bacteria)