Literature summary extracted from
Hsiao, Y.M.; Liu, Y.F.; Fang, M.C.; Tseng, Y.H.
Transcriptional regulation and molecular characterization of the manA gene encoding the biofilm dispersing enzyme mannan endo-1,4-beta-mannosidase in Xanthomonas campestris (2010), J. Agric. Food Chem., 58, 1653-1663.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.2.1.78 |
2-mercaptoethanol |
1 mMm, 121% of initial activity |
Xanthomonas campestris |
|
3.2.1.78 |
Triton X-100 |
1%, 123% of initial activity |
Xanthomonas campestris |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.78 |
expression in Escherichia coli |
Xanthomonas campestris |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.2.1.78 |
additional information |
mutant MG17 is catalytically inactive, mutant protein is present at wild-type molecular mass |
Xanthomonas campestris |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.78 |
Ag+ |
1 mM, 18% residual activity |
Xanthomonas campestris |
|
3.2.1.78 |
Al3+ |
1 mM, 31% residual activity |
Xanthomonas campestris |
|
3.2.1.78 |
Cu2+ |
1 mM, 2% residual activity |
Xanthomonas campestris |
|
3.2.1.78 |
Fe3+ |
1 mM, 37% residual activity |
Xanthomonas campestris |
|
3.2.1.78 |
Hg2+ |
1 mM, 1% residual activity |
Xanthomonas campestris |
|
3.2.1.78 |
Zn2+ |
1 mM, 16% residual activity |
Xanthomonas campestris |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.2.1.78 |
Ca2+ |
1 mM, 119% of initial activity |
Xanthomonas campestris |
|
3.2.1.78 |
Mg2+ |
1 mM, 118% of initial activity |
Xanthomonas campestris |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.78 |
33000 |
- |
x * 33000, SDS-PAGE and calculated |
Xanthomonas campestris |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.78 |
Xanthomonas campestris |
Q8P9S0 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.78 |
recombinant enzyme |
Xanthomonas campestris |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.78 |
? |
x * 33000, SDS-PAGE and calculated |
Xanthomonas campestris |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.78 |
37 |
- |
- |
Xanthomonas campestris |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.78 |
7 |
- |
- |
Xanthomonas campestris |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
3.2.1.78 |
Xanthomonas campestris |
isoform manA transcription is positively regulated by Clp and RpfF and induced by locust bean gum |
up |