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Literature summary extracted from
- Characterization of an antifungal and cryoprotective class I chitinase from table grape berries (Vitis vinifera cv. Cardinal) (2009), J. Agric. Food Chem., 57, 8893-8900.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | expression in Escherichia coli | Vitis vinifera |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Vitis vinifera | Q546P8 | cv. Cardinal | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | recombinant protein, from inclusion bodies | Vitis vinifera |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | skin | - |
Vitis vinifera | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 5.36 | - |
37°C | Vitis vinifera |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | carboxymethylated-chitin-remazol brilliant violet + H2O | - |
Vitis vinifera | ? | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | Chit1b | - |
Vitis vinifera |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 50 | - |
- |
Vitis vinifera |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | - |
50 | more than 50% of maximum activity | Vitis vinifera |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 6 | - |
- |
Vitis vinifera |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4.5 | 8 | more than 50% of maximum activity within | Vitis vinifera |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | physiological function | enzyme exhibits in vitro antifungal activity against Botrytis cinerea and is able to cryoprotect lactate dehydrogenase against freeze/thaw inactivation | Vitis vinifera |
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Release 2023.1 (January 2023)
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