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Literature summary extracted from

  • Silva, M.C.; Ribeiro, A.F.; Terra, W.R.; Ferreira, C.
    Sequencing of Spodoptera frugiperda midgut trehalases and demonstration of secretion of soluble trehalase by midgut columnar cells (2009), Insect Mol. Biol., 18, 769-784.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28 isozyme SfTre1, cDNA library preparation, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, expression in Escherichia coli strain BL21(DE3) Spodoptera frugiperda
3.2.1.28 isozyme SfTre2, cDNA library preparation, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, expression in Escherichia coli strain BL21(DE3) Spodoptera frugiperda

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.47
-
 alpha,alpha-trehalose isozyme SfTre1 Spodoptera frugiperda
3.2.1.28 1.6
-
 alpha,alpha-trehalose isozyme SfTre2 Spodoptera frugiperda

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 membrane bound, isozyme SfTre2 Spodoptera frugiperda 16020
-
3.2.1.28 additional information immunohistochemic localization study of isozyme SfTre1 Spodoptera frugiperda
-
-
3.2.1.28 soluble isozyme SfTre1 Spodoptera frugiperda
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 67000
-
 x * 67000, isozyme SfTre1, SDS-PAGE Spodoptera frugiperda

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O Spodoptera frugiperda
-
 2 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Spodoptera frugiperda B5ATV4 membrane-bound trehalase SfTre2; membrane-bound isozyme SfTre2
-
3.2.1.28 Spodoptera frugiperda Q0ZIF5 soluble trehalase SfTre1; soluble isozyme SfTre1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein membrane-bound trehalase.SfTre2 contains potential N-glycosylation sites Spodoptera frugiperda
3.2.1.28 glycoprotein soluble trehalase.SfTre1 contains potential N-glycosylation sites Spodoptera frugiperda

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 columnar cell midgut Spodoptera frugiperda
-
3.2.1.28 columnar cell midgut, isozyme SfTre1 is localized at the secretory vesicles and at the glycocalyx of columnar cells Spodoptera frugiperda
-
3.2.1.28 larva
-
 Spodoptera frugiperda
-
3.2.1.28 midgut columnar cells, distribution of membrane-bound trehalase, isozyme SfTre2, along Spodoptera frugiperda midgut cells, overview Spodoptera frugiperda
-
3.2.1.28 midgut columnar cells, distribution of soluble trehalase, isozyme SfTre1, along Spodoptera frugiperda midgut cells, overview Spodoptera frugiperda
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Spodoptera frugiperda 2 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 ? x * 67000, isozyme SfTre1, SDS-PAGE Spodoptera frugiperda

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 Alpha,alpha-trehalose glucohydrolase
-
 Spodoptera frugiperda
3.2.1.28 membrane-bound SfTre2
-
 Spodoptera frugiperda
3.2.1.28 SfTre1
-
 Spodoptera frugiperda
3.2.1.28 soluble trehalase
-
 Spodoptera frugiperda

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 62
-
 solubilized membrane-bound isozyme SfTre2, half-life is 10 min Spodoptera frugiperda
3.2.1.28 62
-
 soluble isozyme SfTre1, completely stable Spodoptera frugiperda