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Literature summary extracted from

  • Schenk, M.; Koppisetty, C.A.; Santos, D.C.; Carmona, E.; Bhatia, S.; Nyholm, P.G.; Tanphaichitr, N.
    Interaction of arylsulfatase-A (ASA) with its natural sulfoglycolipid substrates: a computational and site-directed mutagenesis study (2009), Glycoconj. J., 26, 1029-1045.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.8 expression of wild-type and mutant enzymes in CHO-K1 cells Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.8 C69A site-directed mutagenesis, the mnutant shows abolished enzyme activity and only residual binding to thr sulfoglycolipid substrate Sus scrofa
3.1.6.8 C69A/K123A/K302A site-directed mutagenesis, the mutant shows abolished substrate binding and activity Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.8 4-methylumbelliferyl phosphate
-
 Homo sapiens
3.1.6.8 4-methylumbelliferyl phosphate
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.8 acrosome
-
 Homo sapiens
-
-
3.1.6.8 acrosome
-
 Sus scrofa
-
-
3.1.6.8 lysosome
-
 Homo sapiens 5764
-
3.1.6.8 lysosome
-
 Sus scrofa 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.8 sulfogalactosylceramide + H2O Homo sapiens
-
 ?
-
 ?
3.1.6.8 sulfogalactosylceramide + H2O Sus scrofa
-
 ?
-
 ?
3.1.6.8 sulfogalactosylglycerolipid + H2O Homo sapiens
-
 ?
-
 ?
3.1.6.8 sulfogalactosylglycerolipid + H2O Sus scrofa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.8 Homo sapiens P15289
-
-
3.1.6.8 Sus scrofa Q8WNR3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.8 placenta
-
 Homo sapiens
-
3.1.6.8 sperm
-
 Homo sapiens
-
3.1.6.8 testis
-
 Homo sapiens
-
3.1.6.8 testis
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.8 4-nitrocatechol sulfate + H2O artificial substrate, Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview Homo sapiens 4-nitrocatechol + sulfate
-
 ?
3.1.6.8 4-nitrocatechol sulfate + H2O artificial substrate, Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview Sus scrofa 4-nitrocatechol + sulfate
-
 ?
3.1.6.8 additional information substrate binding and docking study, using crystal structures, PDB IDs 1N2K and 1E2S, overview Homo sapiens ?
-
 ?
3.1.6.8 additional information substrate binding and docking study, using human crystal structures, PDB IDs 1N2K and 1E2S, overview Sus scrofa ?
-
 ?
3.1.6.8 sulfogalactosylceramide + H2O
-
 Homo sapiens ?
-
 ?
3.1.6.8 sulfogalactosylceramide + H2O
-
 Sus scrofa ?
-
 ?
3.1.6.8 sulfogalactosylceramide + H2O Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview Homo sapiens ?
-
 ?
3.1.6.8 sulfogalactosylceramide + H2O Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview Sus scrofa ?
-
 ?
3.1.6.8 sulfogalactosylglycerolipid + H2O
-
 Homo sapiens ?
-
 ?
3.1.6.8 sulfogalactosylglycerolipid + H2O
-
 Sus scrofa ?
-
 ?
3.1.6.8 sulfogalactosylglycerolipid + H2O Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview Homo sapiens ?
-
 ?
3.1.6.8 sulfogalactosylglycerolipid + H2O Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.8 arylsulfatase-A
-
 Homo sapiens
3.1.6.8 arylsulfatase-A
-
 Sus scrofa
3.1.6.8 ASA
-
 Homo sapiens
3.1.6.8 ASA
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.8 37
-
 assay at Homo sapiens
3.1.6.8 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.8 5
-
-
 Homo sapiens
3.1.6.8 5
-
-
 Sus scrofa