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Literature summary extracted from

  • Terada, K.; Yamada, J.; Hayashi, Y.; Wu, Z.; Uchiyama, Y.; Peters, C.; Nakanishi, H.
    Involvement of cathepsin B in the processing and secretion of interleukin-1beta in chromogranin A-stimulated microglia (2010), Glia, 58, 114-124.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.36 cathepsin B cathepsin B can effectively cleave and activate pro-caspase-1 in a cell-free system only at an acidic pH and in THP-1 monocytic cells after stimulation with the microbial toxin nigericin Mus musculus
3.4.22.36 chromogranin A chromogranin A can activate pro-caspase-1 in microglia Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.1 additional information cathepsin B deficiency inhibits pro-interleukin-1beta processing and maturation in microglia, no observable expression of the cleaved IL-1beta in the hippocampus of the aged CB-/- mice Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 CA-074-Me a specific inhibitor, inhibits pro-interleukin-1beta processing in microglia Mus musculus
3.4.22.1 E-64 a broad cysteine protease inhibitor Mus musculus
3.4.22.36 CA-074Me the caspase-1 activation is inhibited by CA-074Me, marked inhibition at 0.05 mM Mus musculus
3.4.22.36 E-64d the caspase-1 activation is inhibited by E-64d, marked inhibition at 0.05 mM Mus musculus
3.4.22.36 Z-L-Tyr-L-Val-L-Ala-L-Asp-fluoromethylketone specific inhibitor for caspase-1, complete inhibition at 0.05 mM Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.1 lysosome
-
 Mus musculus 5764
-
3.4.22.1 additional information subcellular localization study, overview Mus musculus
-
-
3.4.22.36 lysosome
-
 Mus musculus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 26000
-
 x * 29000, mature cathepsin B, single-chain form, SDS-PAGE, x * 26000, mature cathepsin B, heavy-chain, SDS-PAGE Mus musculus
3.4.22.1 29000
-
 x * 29000, mature cathepsin B, single-chain form, SDS-PAGE, x * 26000, mature cathepsin B, heavy-chain, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Mus musculus
-
 C57/Bl mice
-
3.4.22.1 Mus musculus C57/Bl
-
 C57/Bl mice
-
3.4.22.36 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 brain
-
 Mus musculus
-
3.4.22.1 microglia isolated from the mixed primary cell cultures from the cerebral cortex of 3-day-old wild-type or CB-/- mice, activated by chromogranin A Mus musculus
-
3.4.22.36 microglia
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.36 pro-interleukin-1beta + H2O
-
 Mus musculus mature interleukin-1beta + ?
-
 ?
3.4.22.36 pro-interleukin-1beta + H2O
-
 Mus musculus C57/Bl mature interleukin-1beta + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.1 ? x * 29000, mature cathepsin B, single-chain form, SDS-PAGE, x * 26000, mature cathepsin B, heavy-chain, SDS-PAGE Mus musculus

Expression

EC Number Organism Comment Expression
3.4.22.1 Mus musculus chromogranin A markedly induces cathepsin B at both protein and mRNA levels, determined by real-time quantitative RT-PCR up

General Information

EC Number General Information Comment Organism
3.4.22.1 physiological function cathepsin B is required for interleukin-1beta maturation, the maturation of pro-IL-1beta in chromogranin A-stimulated microglia is dependent on the enzymatic activities of both cathepsin B and caspase-1 Mus musculus