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Literature summary extracted from

  • Krykbaev, R.; Fitz, L.J.; Reddy, P.S.; Winkler, A.; Xuan, D.; Yang, X.; Fleming, M.; Wolf, S.F.
    Evolutionary and biochemical differences between human and monkey acidic mammalian chitinases (2010), Gene, 452, 63-71.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 methylallosamidin
-
 Homo sapiens
3.2.1.14 methylallosamidin
-
 Macaca fascicularis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.007
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Macaca fascicularis
3.2.1.14 0.032
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 52200
-
 x * 52200, calculated Macaca fascicularis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Homo sapiens
-
-
-
3.2.1.14 Macaca fascicularis D3GG13
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 proteolytic modification sequence contains a hydrophobic leader sequence of 21 amino acids Macaca fascicularis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 stomach high expression of isoform CHIA, no expression of pseudogene CHIA-L1 Homo sapiens
-
3.2.1.14 stomach high expression of isoform CHIA-L1, no expression of isoform CHIA Macaca fascicularis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
-
 Homo sapiens 4-methylumbelliferol + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
-
 Macaca fascicularis 4-methylumbelliferol + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 4-methylumbelliferyl chitobioside + H2O
-
 Homo sapiens 4-methylumbelliferone + chitobiose
-
 ?
3.2.1.14 4-methylumbelliferyl chitobioside + H2O
-
 Macaca fascicularis 4-methylumbelliferone + chitobiose
-
 ?
3.2.1.14 carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.14 carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
-
 Macaca fascicularis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 52200, calculated Macaca fascicularis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 MACase
-
 Macaca fascicularis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 3
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Homo sapiens
3.2.1.14 31.8
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Macaca fascicularis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Homo sapiens
3.2.1.14 5
-
-
 Macaca fascicularis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 2
-
 20% of maximum activity Macaca fascicularis
3.2.1.14 2
-
 30% of maximum activity Homo sapiens
3.2.1.14 3.5
-
 20% of maximum activity Macaca fascicularis
3.2.1.14 3.5
-
 30% of maximum activity Homo sapiens
3.2.1.14 6.5
-
 20% of maximum activity Macaca fascicularis
3.2.1.14 6.5
-
 30% of maximum activity Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.14 0.0000057
-
 pH 5.0, 25°C Homo sapiens methylallosamidin
3.2.1.14 0.000054
-
 pH 5.0, 25°C Macaca fascicularis methylallosamidin

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.14 0.09
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Homo sapiens
3.2.1.14 4.5
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Macaca fascicularis