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Literature summary extracted from

  • Chatterjee, S.; Ehrenshaft, M.; Bhattacharjee, S.; Deterding, L.; Bonini, M.; Corbett, J.; Kadiiska, M.; Tomer, K.; Mason, R.
    Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice (2009), Free Radic. Biol. Med., 46, 454-461.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.17.2 allopurinol addition of allopurinol alone and allopurinol plus 5,5-dimethyl-1-pyrroline N-oxide results in increased CPB1 activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.2 lipopolysaccharide lipopolysaccharide treatment significantly decreases carboxypeptidase B activity. Administration of 5,5-dimethyl-1-pyrroline N-oxide to LPS-treated mice alleviates this loss of enzyme activity Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.2 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.17.2 nitrosylation a post-translational DMPO-nitrone adduct formed with CPB1 in mice treated with a single bolus dose of lipopolysaccharide is detected Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.2 spleen
-
 Mus musculus
-

Synonyms

EC Number Synonyms Comment Organism
3.4.17.2 Carboxypeptidase B1
-
 Mus musculus

General Information

EC Number General Information Comment Organism
3.4.17.2 metabolism nitrone spin-trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and a combination of immuno-spin trapping and mass spectrometry is used to identify in vivo products of radical reactions in mice. Dose-dependent production of 5,5-dimethyl-1-pyrroline N-oxide-CPB1 adducts are detected in the spleens of mice treated with lipopolysaccharide and also under normal physiological conditions. Treatments with inhibitors and experiments with knock-out mice indicate that xanthine oxidase and endothelial nitric oxide synthase are important sources of the reactive species that lead to CPB1 adduct formation Mus musculus