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Literature summary extracted from

  • He, S.; Bystricky, K.; Leon, S.; Francois, J.M.; Parrou, J.L.
    The Saccharomyces cerevisiae vacuolar acid trehalase is targeted at the cell surface for its physiological function (2009), FEBS J., 276, 5432-5446.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28 expression of GFP-tagged Ath1 N-terminal domain Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.28 additional information complementation of the Saccharomyces cerevisiae suc2D mutant SEY6210 strain with different Ath1-invertase chimera, where the Suc2 signal peptide is replaced by full-length ATH1 sequence, overview. Hybrid Ath1 truncated mutants fused at their C-terminus with the yeast internal invertase reveal that a 131 amino acid N-terminal fragment of Ath1 is sufficient to target the fusion protein to the cell surface, enabling growth of the suc2DELTA mutant on sucrose. Removal of the N-terminus of Ath1 causes a strict vacuolar localization. Fusion of the signal peptide of invertase to N-terminally truncated Ath1 allows the ath1D mutant to grow on trehalose, whereas the signal sequence of the vacuolar-targeted Pep4 constrained Ath1 in the vacuole and prevents growth of this mutant on trehalose Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 cell surface Ath1 Saccharomyces cerevisiae 9986
-
3.2.1.28 additional information Ath1 possesses a transmembrane domain. Existence of a secretion sequence in Ath1 Saccharomyces cerevisiae
-
-
3.2.1.28 vacuole Ath1 Saccharomyces cerevisiae 5773
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 30000
-
 x * 30000, about, recombinant GFP-tagged Ath1 N-terminal domain Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O Saccharomyces cerevisiae trehalose is one of the major storage carbohydrates in the yeast Saccharomyces cerevisiae 2 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Saccharomyces cerevisiae
-
 neutral Nth1 and Nth2 and acid Ath1, encoded by gene ath1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Saccharomyces cerevisiae 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O trehalose is one of the major storage carbohydrates in the yeast Saccharomyces cerevisiae Saccharomyces cerevisiae 2 D-glucose
-
 ?
3.2.1.28 additional information the 131 amino acid N-terminus of Ath1 are sufficient for invertase secretion, and the short transmembrane domain, located at the N-terminus, is indispensable for Ath1 function, overview Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 ? x * 30000, about, recombinant GFP-tagged Ath1 N-terminal domain Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 Ath1
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 4.5
-
 Ath1 Saccharomyces cerevisiae