EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.4 | sodium taurodeoxycholate | maximum phospholipase activity at 1 mm sodium taurodeoxycholate. Displays 50% of its maximum activity in the absence of bile salt dependent | Gallus gallus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.4 | ligated into PCR-Blunt vector and transformed into Escherichia coli DH5alpha cells | Gallus gallus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.4 | Ca2+ | essential for both catalysis and enzyme binding to the substrate. The catalytic Ca2+ may be coordinated in a tetrahedral environment formed by the oxygen atoms of Tyr28, Gly30, Gly32 and Asp49. Second Ca2+-binding site is conserved and is formed by four residues: Glu71, Ile72, Asn89, and Glu92. Optimal Ca2+ concentration is about 4 mM | Gallus gallus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 14000 | - |
gel filtration | Gallus gallus |
3.1.1.4 | 14170 | - |
calculated from sequence | Gallus gallus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.4 | Gallus gallus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.4 | by ammonium sulfate and ethanol precipitation, followed by gel filtration and gel filtration, to homogeneity, with a yield of 16%, 2000fold | Gallus gallus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.4 | pancreas | - |
Gallus gallus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 0.2 | - |
extraction at pH 8.5, in 10 mM Tris/HCl buffer, 10 mM CaCl2, 0.15 M NaCl | Gallus gallus |
3.1.1.4 | 400 | - |
2000fold purified enzyme, at pH 9.5, 37°C, in the presence of 1 mM sodium taurodeoxycholate, 4 mM CaCl2 with phosphatidylcholine as substrate | Gallus gallus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.4 | phosphatidylcholine + H2O | in the presence of substrate, the enzyme binds to the interface as an active form. This crucial step is mediated by a planar surface region of the protein. The corresponding hydrophobic surface involves hydrophobic residues (Leu2, Trp3, Phe19, Leu20, Leu31, Ile64, Leu65, Tyr69, Ile72, Tyr75, and Leu118), hydrophobic surface involved in lipid binding increases | Gallus gallus | 1-acylglycerophosphocholine + a carboxylate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.4 | monomer | non-glycosylated protein, SDS-PAGE | Gallus gallus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.4 | group IB PLA2 | - |
Gallus gallus |
3.1.1.4 | phospholipase A2 | - |
Gallus gallus |
3.1.1.4 | PLA2 | - |
Gallus gallus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 37 | - |
with phosphatidylcholine as substrate in the presence of 4 mM Ca2+ and 1 mM sodium taurodeoxycholate | Gallus gallus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 37 | 40 | loses ca. 80% of its activity after 20 min of incubation at 40°C | Gallus gallus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 9.5 | - |
with phosphatidylcholine as substrate in the presence of 4 mM Ca2+ and 1 mM sodium taurodeoxycholate | Gallus gallus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 4 | 9.5 | - |
Gallus gallus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 4 | - |
is inactivated after incubation for 30 min at pH values lower than 4 | Gallus gallus |