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Literature summary extracted from

  • Guo, Y.; Bandaru, V.; Jaruga, P.; Zhao, X.; Burrows, C.J.; Iwai, S.; Dizdaroglu, M.; Bond, J.P.; Wallace, S.S.
    The oxidative DNA glycosylases of Mycobacterium tuberculosis exhibit different substrate preferences from their Escherichia coli counterparts (2010), DNA Repair, 9, 177-190.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.27 additional information the interaction between Lhr and MtuNei2 may be crucial for recruiting MtuNei2 onto damaged DNA and stimulating the activity of MtuNei2 during DNA repair Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 genes Rv2464c and Rv3297, expression of His-tagged enzymes from a bicistronic vector in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.27 additional information nei nth double mutants exhibit an elevated spontaneous mutation frequency. Spontaneous mutations observed in the double mutant are solely from C to T transitions due to the inability to repair oxidized cytosines, which can be efficiently prevented by MtuNei1 and MtuNei2 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.27 additional information
-
 additional information kinetics of MtuNei1 with diverse substrates, overview Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.27 28500
-
 x * 28500, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 additional information Mycobacterium tuberculosis MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea ?
-
 ?
3.2.2.27 additional information Mycobacterium tuberculosis H37Rv MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Mycobacterium tuberculosis
-
 genes Rv2464c and Rv3297, encoding MtuNei1 and MtuNei2
-
3.2.2.27 Mycobacterium tuberculosis H37Rv
-
 genes Rv2464c and Rv3297, encoding MtuNei1 and MtuNei2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 recombinant His-tagged enzymes from Escherichia coli by nickel affinity chromatograpyh Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 additional information MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea Mycobacterium tuberculosis ?
-
 ?
3.2.2.27 additional information MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei1 excises thymine glycol and strongly prefers the 5R isomers. GC/MS analysis of products released by MtuNei1, overview. Substrates are oligonucleotides with 7,8-dihydro-8-oxoguanine, thymine glycol, 5,6-dihydrouracil, 5,6-dihydrothymine, 5-hydroxyuracil, 5-hydroxycytosine, uracil, and furan Mycobacterium tuberculosis ?
-
 ?
3.2.2.27 additional information MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea Mycobacterium tuberculosis H37Rv ?
-
 ?
3.2.2.27 additional information MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei1 excises thymine glycol and strongly prefers the 5R isomers. GC/MS analysis of products released by MtuNei1, overview. Substrates are oligonucleotides with 7,8-dihydro-8-oxoguanine, thymine glycol, 5,6-dihydrouracil, 5,6-dihydrothymine, 5-hydroxyuracil, 5-hydroxycytosine, uracil, and furan Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.27 ? x * 28500, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 endonuclease VIII
-
 Mycobacterium tuberculosis
3.2.2.27 MtuNei1
-
 Mycobacterium tuberculosis
3.2.2.27 UDG
-
 Mycobacterium tuberculosis
3.2.2.27 uracil DNA glycosylase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.27 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.27 8
-
 assay at Mycobacterium tuberculosis