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Literature summary extracted from

  • Stefansson, B.; Helgadottir, L.; Olafsdottir, S.; Gudmundsdottir, A.; Bjarnason, J.B.
    Characterization of cold-adapted Atlantic cod (Gadus morhua) trypsin I--kinetic parameters, autolysis and thermal stability (2010), Comp. Biochem. Physiol. B, 155, 186-194.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.4 trypsin I DNA and amino acid sequence determination and analysis Gadus morhua

General Stability

EC Number General Stability Organism
3.4.21.4 autolysis is a contributing factor in the stability of trypsin I Gadus morhua

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 3,4-dichloroisocoumarin
-
 Gadus morhua
3.4.21.4 Nalpha-tosyl-L-Lys-chloromethylketone
-
 Gadus morhua
3.4.21.4 PMSF
-
 Gadus morhua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.007
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 0.009
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 9.0, 25°C Gadus morhua
3.4.21.4 0.013
-
 MeOSuc-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 0.015
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 7.1, 25°C Gadus morhua
3.4.21.4 0.018
-
 MeOSuc-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 0.027
-
 MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 0.034
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 6.0, 25°C Gadus morhua
3.4.21.4 0.05
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Gadus morhua

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Gadus morhua
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 native trypsin I by benzamidine affinity and anion exchange chromatography Gadus morhua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide + H2O
-
 Gadus morhua MeOSuc-Ala-Ala-Ala-Arg + 4-nitroaniline
-
 ?
3.4.21.4 MeOSuc-Ala-Ala-Arg 4-nitroanilide + H2O
-
 Gadus morhua MeOSuc-Ala-Ala-Arg + 4-nitroaniline
-
 ?
3.4.21.4 MeOSuc-Ala-Arg 4-nitroanilide + H2O
-
 Gadus morhua MeOSuc-Ala-Arg + 4-nitroaniline
-
 ?
3.4.21.4 additional information identification of autolytic cleavage sites within the trypsin I molecule, overview Gadus morhua ?
-
 ?
3.4.21.4 N-benzoyl-DL-arginine 4-nitroanilide + H2O
-
 Gadus morhua N-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
3.4.21.4 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O
-
 Gadus morhua N-benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 Atlantic cod trypsin I
-
 Gadus morhua
3.4.21.4 trypsin I
-
 Gadus morhua

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.4 additional information
-
 Atlantic cod trypsin I is a highly cold-adapted enzyme Gadus morhua
3.4.21.4 25
-
 assay at Gadus morhua

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.4 additional information
-
 Gadus morhua trypsin I is less stable towards thermal unfolding than its mesophilic bovine analogue Gadus morhua
3.4.21.4 15 37 purified enzyme, activity is lost relatively rapidly at 37 °C with a half-life of 17 h, at 25 °C the half-life is 5.4 days, while at 15 °C the enzyme is much more stable with a half-life of 32.5 days Gadus morhua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 1.34
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 18.3
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 6.0, 25°C Gadus morhua
3.4.21.4 24.9
-
 MeOSuc-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 30.5
-
 MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 33.6
-
 MeOSuc-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 48.9
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 9.0, 25°C Gadus morhua
3.4.21.4 53.1
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 54.5
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 7.1, 25°C Gadus morhua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 8
-
-
 Gadus morhua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.4 26.8
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 538.2
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 6.0, 25°C Gadus morhua
3.4.21.4 1130
-
 MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 1383
-
 MeOSuc-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 2585
-
 MeOSuc-Ala-Ala-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua
3.4.21.4 3759
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 7.1, 25°C Gadus morhua
3.4.21.4 5433
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 9.0, 25°C Gadus morhua
3.4.21.4 7590
-
 N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide pH 8.0, 25°C Gadus morhua