EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.4 | Aprotinin | - |
Sitophilus zeamais | |
3.4.21.4 | benzamidine | - |
Sitophilus zeamais | |
3.4.21.4 | Bowman-Birk inhibitor | - |
Sitophilus zeamais | |
3.4.21.4 | E64 | weak inhibition | Sitophilus zeamais | |
3.4.21.4 | leupeptin | - |
Sitophilus zeamais | |
3.4.21.4 | N-alpha-tosyl-L-lysine chloromethyl ketone | - |
Sitophilus zeamais | |
3.4.21.4 | pepstatin | - |
Sitophilus zeamais | |
3.4.21.4 | phenylmethyl sulfonyl fluoride | - |
Sitophilus zeamais | |
3.4.21.4 | Soybean trypsin inhibitor | - |
Sitophilus zeamais |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.4 | additional information | - |
additional information | kinetics | Sitophilus zeamais |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.4 | Ca2+ | activates, best at 20 mM | Sitophilus zeamais |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.4 | Sitophilus zeamais | - |
insecticide-resistant and -susceptible strains, serine proteinases with trypsin-like activity | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.4 | native enzymes partially by aprotinin affinity chromatography, 51.2fold | Sitophilus zeamais |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.4 | 529 | 875 | partially purified native trypsin-like serine proteinases, pH 8.2, 25°C | Sitophilus zeamais |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.4 | casein + H2O | - |
Sitophilus zeamais | ? | - |
? | |
3.4.21.4 | N-alpha-p-tosyl-L-Arg methyl ester + H2O | - |
Sitophilus zeamais | N-alpha-p-tosyl-L-Arg + methanol | - |
? | |
3.4.21.4 | N-benzoyl-L-arginine 4-nitroanilide + H2O | the trypsin-like proteinases from the resistant strains exhibited higher affinity for the substrate than the enzymes from the susceptible strains | Sitophilus zeamais | N-benzoyl-L-arginine + 4-nitroaniline | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.4 | trypsin-like serine proteinase | - |
Sitophilus zeamais |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.4 | 25 | - |
- |
Sitophilus zeamais |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.4 | 10 | 65 | assay range | Sitophilus zeamais |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.4 | 8.2 | - |
- |
Sitophilus zeamais |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.4 | 4 | 10 | assay range | Sitophilus zeamais |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.21.4 | additional information | enhanced serine proteinase activity may be playing a role in mitigating physiological costs associated with the maintenance of insecticide resistance mechanisms in some maize weevil strains | Sitophilus zeamais |