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Literature summary extracted from

  • Silva, L.B.; Reis, A.P.; Pereira, E.J.; Oliveira, M.G.; Guedes, R.N.
    Partial purification and characterization of trypsin-like proteinases from insecticide-resistant and -susceptible strains of the maize weevil, Sitophilus zeamais (2010), Comp. Biochem. Physiol. B, 155, 12-19.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 Aprotinin
-
Sitophilus zeamais
3.4.21.4 benzamidine
-
Sitophilus zeamais
3.4.21.4 Bowman-Birk inhibitor
-
Sitophilus zeamais
3.4.21.4 E64 weak inhibition Sitophilus zeamais
3.4.21.4 leupeptin
-
Sitophilus zeamais
3.4.21.4 N-alpha-tosyl-L-lysine chloromethyl ketone
-
Sitophilus zeamais
3.4.21.4 pepstatin
-
Sitophilus zeamais
3.4.21.4 phenylmethyl sulfonyl fluoride
-
Sitophilus zeamais
3.4.21.4 Soybean trypsin inhibitor
-
Sitophilus zeamais

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 additional information
-
additional information kinetics Sitophilus zeamais

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.4 Ca2+ activates, best at 20 mM Sitophilus zeamais

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Sitophilus zeamais
-
insecticide-resistant and -susceptible strains, serine proteinases with trypsin-like activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 native enzymes partially by aprotinin affinity chromatography, 51.2fold Sitophilus zeamais

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.4 529 875 partially purified native trypsin-like serine proteinases, pH 8.2, 25°C Sitophilus zeamais

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 casein + H2O
-
Sitophilus zeamais ?
-
?
3.4.21.4 N-alpha-p-tosyl-L-Arg methyl ester + H2O
-
Sitophilus zeamais N-alpha-p-tosyl-L-Arg + methanol
-
?
3.4.21.4 N-benzoyl-L-arginine 4-nitroanilide + H2O the trypsin-like proteinases from the resistant strains exhibited higher affinity for the substrate than the enzymes from the susceptible strains Sitophilus zeamais N-benzoyl-L-arginine + 4-nitroaniline
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 trypsin-like serine proteinase
-
Sitophilus zeamais

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.4 25
-
-
Sitophilus zeamais

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.4 10 65 assay range Sitophilus zeamais

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 8.2
-
-
Sitophilus zeamais

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.4 4 10 assay range Sitophilus zeamais

General Information

EC Number General Information Comment Organism
3.4.21.4 additional information enhanced serine proteinase activity may be playing a role in mitigating physiological costs associated with the maintenance of insecticide resistance mechanisms in some maize weevil strains Sitophilus zeamais